长沙市首例人感染H9N2禽流感病毒的HA基因序列特征分析  

Isolation and characteristic analysis of an avian influenza virus(H9N2)from a patient in Changsha,China

作　　者：黄政[1] 欧新华[1] 袁洁[1] 刘晓蕾[1] 

机构地区：[1]长沙市疾病预防控制中心,长沙410004

出　　处：《中国人兽共患病学报》2016年第11期997-1000,共4页Chinese Journal of Zoonoses

摘　　要：目的对长沙市首例人源H9N2禽流感病毒进行病毒分离与HA基因特征序列分析。方法用细胞培养法在可疑病例标本中分离H9N2禽流感病毒毒株,RT-PCR方法扩增禽流感病毒HA基因全长并测序,对所得结果进行氨基酸同源性与进化树分析。结果分离的毒株命名为A/Hunan/44558/2015,GenBank登录号为KX595338。毒株存在2处新发突变位点,HAT197D、HAD483N,第313位出现与湖南省人源H9N2毒株A/Lengshuitan/11197/2013相同的糖基化位点NCS。与A/Wild Chicken/Shanghai/C1/2014和A/Environment/Hunan/28028/2014的同源性分别为97.9%和97.6%。进化树分析分离的毒株处于Y280分支。结论发现病毒2处新的基因突变位点,与2014年湖南省环境源毒株同源性较高,存在基因交换重组,从分子水平揭示病毒HA基因的进化趋势。In order to analyze the characteristic of hemagglutinin gene in an avian influenza virus（H9N2）that occurred in human,influenza-like illness sample was collected in a routine supervision and we confirmed the virus as H9N2 avian influenza virus by RT-PCR,and DNA sequencing.Homology and phylogeny tree analysis was performed after amplification of the HA gene fragment.The nucleotide sequence GenBank accession number is NO：KX595338.DNA sequencing showed that two mutation sites included HAT197 Dand HAD483N were found.The glycosylation site in 313 location of HA gene was the same to A/Lengshuitan/11197/2013 virus.Phylogenetic and homology analysis revealed that it belong to Y280-like branch,and had97.6% percent identity compared with A/Environment/Hunan/28028/2014.In all,the study results reveal two novel mutation sites,and with highly homology with avian influenza virus isolated from Hunan Province environment.Our results highlight the genetic re-assortment of H9N2 virus was existed.

关 键 词：禽流感 H9N2 人感染禽流感 血细胞凝集素 进化树分析 

分 类 号：R378.2[医药卫生—病原生物学]