DAS-ELISA与RT-PCR法检测马铃薯S病毒的比较  

Comparison of DAS-ELISA and RT-PCR Method for Detection of Potato Virus S

作　　者：申宇[1] 高艳玲[1] 范国权[1] 张威[1] 张抒[1] 白艳菊[1] 

机构地区：[1]黑龙江省农业科学院植物脱毒苗木研究所/农业部脱毒马铃薯种薯质量监督检验测试中心,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2016年第10期58-60,共3页Heilongjiang Agricultural Sciences

基　　金：现代农业产业技术体系专项资金资助项目(CARS-10-P14);哈尔滨市科技创新人才专项资金资助项目(2015RQQYJ031);国家科技支撑计划资金资助项目(2012BAD06B02)

摘　　要：在马铃薯种薯生产中,马铃薯S病毒是田间发病率较高的病毒之一,同时也是最不易脱掉的病毒。为了更准确地筛查马铃薯病毒病,减少病毒病在田间的侵染,保证种薯质量,提高种薯产量,用DAS-ELISA和RT-PCR两种方法对马铃薯S病毒筛查的效果进行了比较。结果表明:两种方法间有较高的吻合度,但也存在一定的差异。DAS-ELISA相对RT-PCR而言成本更低效率更高,但灵敏度略低。同一条件下,对于同一样本,不同的试验方法会对试验结果有一定的影响。因此,在病毒检测工作中,应根据检测的实际要求,谨慎合理选取正确的方法,灵活利用RT-PCR进行补充检测,以保证检测及科研工作结果更加可靠。Potato virus S （PVS） is one of the viruses which causes high rate of incidence in the seed potatoes production,and it is also the hardest one to free. Therefore,it appears frequently in the fields. In order to screen the potato virus disease more accurately, reduce the infection of virus in the field,ensure the quality of seed potato,increase the seed potato yield, the effect of virus detection by DAS-ELISA and RT-PCR methods were compared and studied. The results showed that two methods had a good agreement ,but there are some differences, too. Compared with RT-PCR, DAS-ELISA was lower costly, higher efficient, but the sensitivity was lower. Different methods in same situations have a certain effect on the experimental results. Therefore,in the virus detection work, correct method should be chosen based on the actual requirements of the detection, carefully and reasonably. RT-PCR method should be used to complement the detection,to ensure that the results of testing and scientific researchwas more reliable.

关 键 词：马铃薯 PVS 检测 方法 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]