白花九里明对急性肝损伤小鼠的保护作用及机制  

Protective effect of Blumea megacephala on acute liver injury in mice

作　　者：刘桂彪[1] 张铭锋[1] 申璐[1] 黄剑秋[1] 郭莹[1] 黄文飞[1] 闭丽华 王保山[1] 王彩冰[1] 方晓燕[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《山东医药》2016年第41期14-17,共4页Shandong Medical Journal

基　　金：2015年地方高校国家级大学生创新创业训练计划项目(201510599009)

摘　　要：目的探讨白花九里明提取液对急性肝损伤小鼠的保护作用及机制。方法取KM小鼠50只,随机均分为5组,正常对照组和模型对照组给予0.9%Na Cl注射液,联苯双酯组给予0.2 g/kg联苯双酯,白花九里明低浓度组和高浓度组分别给予15、30 g/kg的白花九里明提取液灌胃7 d;同时在灌胃的第1、3、5天,对正常对照组给予0.9%Na Cl注射液腹腔注射,模型对照组、联苯双酯组、白花九里明低浓度和高浓度组均给予0.1%四氯化碳(CCl4)溶液腹腔注射。末次灌胃1 h后,采血分离血清、取肝左叶制肝悬液分离上清液、取肝右叶制备组织切片。检测肝匀浆和血清的总蛋白(TP)含量、谷丙转氨酶(GPT)活性、谷草转氨酶(GOT)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,镜下观察肝组织结构变化。结果模型对照组、联苯双酯组、白花九里明低浓度组肝匀浆TP含量明显高于正常对照组(P均<0.01),白花九里明高浓度组肝匀浆TP含量明显高于正常对照组和模型对照组(P均<0.05),正常对照组、模型对照组、白花九里明低浓度组肝匀浆GPT活性明显高于联苯双酯组和白花九里明高浓度组(P均<0.05),白花九里明低浓度组血清GOT活性明显低于正常对照组(P<0.05);白花九里明高浓度组、联苯双酯组、模型对照组肝匀浆SOD活性明显低于正常对照组(P均<0.05),白花九里明低浓度组的肝匀浆SOD活性明显高于联苯双酯组(P<0.05),白花九里明低浓度组血清SOD活性明显高于正常对照组、模型对照组、联苯双酯组、白花九里明高浓度组(P均<0.01);白花九里明低浓度组和高浓度组肝匀浆MDA含量明显低于正常对照组(P均<0.05);其余各组血清TP含量、GPT活性、GOT活性、SOD活性、MDA含量组间两两比较差异无统计学意义(P均>0.05)。镜下示联苯双酯组、白花九里明低浓度组和高浓度组与模型对照组相比,肝组织结构清晰,肝细胞变性溶解坏死数量�Objective To investigate the protective effect of Blumea megacephala extracting solution on acute liver injury of mice and its mechanism of action.Methods Fifty KMmice were randomly divided into 5 groups.Normal con-trol group and model control group were given 0.9% NaCl injection,bifendatatum group was given 0.2 g/kg bifendatatum, Blumea megacephala low-concentration group and high-concentration group were respectively given 15 and 30 g/kg Blumea megacephala extracting solution for 7 days by gastric irrigation.Meanwhile,on day 1,3 and 5 of gastric irrigation,the nor-mal control group was given 0.9% NaCl by intraperitoneal injection,the rest of the groups were given 0.1%Carbon tetra-chloride （CCL4 ）liquid.One hour 1 h after the last intragastric administration,we collected and separated the serum,and＆nbsp;took the left lobe of liver to make and separate the liver supernatant,took the right lobe of liver to make tissue section.The total protein （TP）,glutamic-pyruvic transaminase （GPT）,glutamic-oxalacetic transaminase （GOT）,superoxide dis-mutase （SOD）and malondialdehyde （MDA）content or activity in liver homogenate and serum were detected and we ob-served the structure changes of liver tissue microscopically.Results The TP content of liver homogenate in the model con-trol group,bifendatatum group and Blumea megacephala low-concentration group was obviously higher than that of the nor-mal control group （all P 〈0.01）.The TP content of liver homogenate in the Blumea megacephala high-concentration group was higher than that of the normal control group and model control group （all P 〈0.05）.The GPT activity of liver homoge-nate in the normal control group,model control group and Blumea megacephala low-concentration group was higher than that of the bifendatatum group and Blumea megacephala high-concentration group （all P 〈0.05）.The GOT activity of ser-um in the Blumea megacephala low-concentration group was lower than that of the normal control group （P 〈0.05）.The SOD activ

关 键 词：急性肝损伤 壮药 白花九里明提取液 谷丙转氨酶活性 谷草转氨酶活性 超氧化物歧化酶活性 丙二醛 小鼠 

分 类 号：R575[医药卫生—消化系统] R931.71[医药卫生—内科学]