甘肃鼢鼠适应低氧的肌纤维类型和代谢特征  

Muscle fiber types and metabolic characteristics of Gansu zokor(Myospalax cansus)in adaptation to hypoxia

作　　者：山萌 王文静[1] 葛宝[1] 李金钢[1] 何建平[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710119

出　　处：《西北农林科技大学学报（自然科学版）》2016年第10期18-24,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30670360)

摘　　要：【目的】研究甘肃鼢鼠(Myospalax cansus)骨骼肌肌纤维类型和代谢特征,为动物适应低氧环境的生理机制研究提供理论依据。【方法】以SD大鼠为对照,应用mATP酶法测定常氧及急、慢性低氧条件下甘肃鼢鼠肌纤维类型及不同类型肌纤维横截面积,用分光光度计法测定甘肃鼢鼠腓肠肌琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)活性和肌红蛋白(Mb)含量。【结果】与常氧组相比,急、慢性低氧对甘肃鼢鼠肌纤维组成及横截面积无显著影响;与SD大鼠相比,甘肃鼢鼠Ⅰ型肌纤维比例极显著降低(P<0.01),Ⅱa型肌纤维比例极显著升高(P<0.01);急、慢性低氧处理后,甘肃鼢鼠SDH、LDH活性较常氧组无显著性变化,但显著低于SD大鼠(P<0.05);甘肃鼢鼠Mb含量与常氧组相比无显著性变化,但显著高于SD大鼠(P<0.05)。【结论】与SD大鼠相比,甘肃鼢鼠的肌纤维类型和代谢特征有明显的低氧适应性。【Objective】The paper studied the hypoxia adaptation of muscle fiber types and metabolic characteristics of Gansu zokor to provide theoretical basis for physiological mechanism of animals to adapt hypoxia environment.【Method】Using SD rats as control,mATPase reaction was used to determine the composition and change of cross-sectional area of different muscle fibers.Spectrophotometry was used to determine the gastrocnemius activity of succinate dehydrogenase（SDH）,lactate dehydrogenase（LDH）and content of myoglobin（Mb）.【Result】Acute and chronic hypoxia had insignificant effects on composition of zokors muscle fibers and cross sectional area.Compared with SD rats,the ratio of muscle fiber typeⅠ of zokors was significantly reduced（P〈0.01）,while that of muscle fiber typeⅡa was significantly increased（P〈0.01）.After acute and chronic hypoxia,activities of SDH and LDH showed insignificant change and were significantly lower than those of SD rats（P〈0.05）.Myoglobin content of zokors had no significant change and was significantly higher than that of SD rats（P〈0.05）.【Conclusion】The muscle fiber types and metabolic characteristics of zokors adapted better to hypoxia than SD rats.

关 键 词：甘肃鼢鼠 低氧 腓肠肌 肌纤维类型 

分 类 号：Q445[生物学—生理学]