异硫氰酸苯酯柱前衍生反相高效液相色谱法测定复序橐吾中21种氨基酸的含量  

Determination of contents of 21 kinds of ammo acids in Ligularia jaluensis by using PITC pre-column derivation RP-HPLC method

作　　者：赵岩[1] 彭蕾[1] 陆珞 郜玉钢[1] 蔡恩博[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林省食品药品审评中心,长春130062

出　　处：《中华中医药杂志》2016年第12期5049-5052,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技支撑计划项目(No.2011BAI03B01);国家公益性行业科研专项(No.201303111);科技部科技人员服务企业项目(No.2009GJB10031);吉林省科技发展计划项(No.20130303094;No.20140204 013YY;No.20140311050YY;No.20150307012YY)~~

摘　　要：目的:测定复序橐吾中游离和水解氨基酸的含量。方法:以异硫氰酸苯酯(PITC)为柱前衍生试剂,用反相高效液相色谱法(RP-HPLC)测定复序橐吾中氨基酸的含量。结果:21种氨基酸的检测浓度线性范围为0.0022-2.1000μmol/L,精密度RSD在0.38%-1.05%之间;稳定性RSD在0.46%-1.28%之间;游离氨基酸和水解氨基酸的重复性RSD在1.20%-2.38%和0.86%-2.43%之间;游离氨基酸和水解氨基酸的加样平均回收率分别在97.89%-101.05%和98.04%-103.03%之间且RSD均小于4%,游离和水解氨基酸的含量分别为0.2307%和8.7943%。结论:复序橐吾中含有21种氨基酸,并确定了其含量。Objective： To determinate the contents of free and hydrolysis amino acids in Ligularia Jaluensis. Methods： The amino acids contents in Ligularia Jaluensis were determined by using reversed phase high-performance liquid chromatography（RH-HPLC） with phenyl isothiocyanate（PITC） as the pre-column derivation reagent. Results： The 21 amino acids had good linearity in the range of 0.0022-2.1 000μmol/L. The RSD of precision was between 0.38% and 1.05%, the RSD of stability was between 0.46% and 1.28%, and the repeatability of free and hydrolysis amino acids was between 1.20% and 2.38%, or between 0.86% and 2.43%. The sample recovery rate of free and hydrolysis amino acids was between 97.89% and 101.05%, or between 98.04 % and 103.03%, and the RSD was less than 4%. The content of free amino acid and hydrolytic amino acid was 0.2307% and 8.7943%. Conclusion： There were 21 amino acids in Ligularia Jaluensis, and the contents of the 21 amino acids were determined.

关 键 词：复序橐吾 氨基酸 异硫氰酸苯酯 柱前衍生 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]