益气养血泻浊法对慢性肾衰竭营养不良大鼠Akt/FoxO1通路的影响  

Effects of replenishing qi, nourishing blood and resolving turbidity method against Akt/Fox O1 pathway in rat model of chronic renal fail with malnutrition

作　　者：邓聪[1] 魏连波[2] 汪东涛[2] 鲁路[2] 赖艳娴[1] 王康玮[1] 

机构地区：[1]广州市第一人民医院南沙医院,广州511457 [2]南方医科大学珠江医院中西医结合肾病中心国家中医药管理局肾病重点学科,广州510282

出　　处：《中华中医药杂志》2016年第12期5228-5231,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173457;No.81373808);广州市医药卫生科技项目(No.20142A011019)~~

摘　　要：目的:观察益气养血泻浊法中药肾衰养真胶囊对慢性肾衰竭(CRF)营养不良大鼠蛋白激酶B(Akt)/叉头转化因子1(Fox O1)信号通路的影响。方法:采用5/6肾切除同时予4%酪蛋白饮食制作CRF营养不良大鼠模型。大鼠随机分为正常组、模型组、中药组和西药组。治疗4周后,检测肌酐(Scr)、尿素氮(BUN)、白蛋白(Alb)、血红蛋白(Hb);蛋白质印迹法(Western Blot)检测骨骼肌磷酸化Akt(p-Akt)蛋白水平;逆转录-实时定量聚合酶链式反应(Real-time PCR)检测骨骼肌肌肉萎缩基因(MAFbx)和肌环指蛋白-1基因(Mu RF-1)和Fox O1 m RNA水平。结果:与模型组比较,中药组大鼠骨骼肌Fox O1 m RNA、MAFbx m RNA和Mu RF-1 m RNA表达水平均显著下降(P<0.01),而p-Akt蛋白表达水平显著上升(P<0.05),同时,大鼠Scr、Alb、Hb均显著上升(P<0.05,P<0.01)。且与西药组比较,中药组Hb显著升高(P<0.01)。结论:益气养血泻浊法中药肾衰养真胶囊能显著改善CRF营养不良大鼠的营养状况,其机制可能通过下调骨骼肌Fox O1 m RNA、MAFbx m RNA和Mu RF-1m RNA表达,上调p-Akt蛋白表达水平,从而减少蛋白分解,改善CRF营养不良。Objective： To investigate the effect of Shenshuai Yangzhen Capsule（SSYZC） on protein kinase B（Akt）/Forkhead box O1（Fox O1） pathway in chronic renal failure（CRF） rats with malnutrition（MN）. Methods： SPF grade male SD rats were suffered a 5/6 nephrectomy and 4% casein diet to establish the CRF-MN rat model. Rats were randomly divided into four groups： control group, model group, Chinese medicine group, western medicine group. After being treated for 4 weeks, the renal function related indexes, including serum creatinine（Scr）, urea nitrogen（BUN）, albumin（Alb）, haemoglobin（Hb） were measured. The expression of phospho-Akt（p-Akt） protein in skeletal muscle was measured by Western Blot. The expression of Fox O1, muscle atrophy F box（MAFbx） and Muscle RING-finger protein 1（Mu RF-1） in skeletal muscle was determined by Real-time PCR. Results： Compared with model group, the gene expressions of Fox Ol, MAFbx and Mu RF-1 in skeletal muscle of rats with MN in Chinese medicine group were significantly down-regulated（P〈0.01）, but the expression of p-Akt protein was markedly upregulated（P〈0.05）. Meanwhile, the levels of Alb and Hb in Chinese medicine group were significantly increased when compared to the model group. Conclusion： SSYZC can improve the malnutrition condition of CRF-MN rat model, which is possibly related to the downregulation of the genes expression of Fox O1 m RNA, MAFbx m RNA and Mu RF-1 m RNA and the upregulation of expression of p-Akt protein in skeletal muscle, resulting in a decrease in the albuminolysis.

关 键 词：益气养血泻浊法 肾衰养真胶囊 慢性肾衰竭 营养不良 蛋白激酶B 叉头转化因子1 

分 类 号：R285.5[医药卫生—中药学]