以贻贝贝壳为原料制备的无定形磷酸钙及其性质  

Synthesis and Properties of Amorphous Calcium Phosphate Microspheres with Mussel Shells

作　　者：韩义忠 崔正阳[2] 陈岑[2] 叶婷[2] 孔祥东[1,2] HAN Yizhong CUI Zhengyang CHEN Cen YE Ting KONG Xiangdong(a. College of Materials and Textile b. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Chin)

机构地区：[1]浙江理工大学材料与纺织学院,杭州310018 [2]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2016年第6期849-854,共6页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(51272236;51002139)

摘　　要：以废弃的贻贝贝壳资源为原料,将贝壳简单粉碎后作为钙源制备出了无定形磷酸钙（ACP）。在反应过程中先后加入磷酸、三聚磷酸钠及尿素,快速制备了直径为250～450nm的球形亚微米颗粒,该颗粒粒径分布范围窄,尺寸均一,表面积大,颗粒表面电位（Zeta电位）为27.5mV。该无定形磷酸钙颗粒与量子点结合性良好,MTT试验表明该无定形磷酸钙颗粒对成骨细胞（MC3T3-E1）不显示毒性,生物相容性良好。Amorphous calcium phosphate （ACP）was successfully synthesized by utilizing the calcium precursor of mussel shell powder.Phosphoric acid,sodium tripolyphosphate and urea were added during the reaction To fast prepare spherical submicron particles with the diameter of 250～450 nm.Particle size distribution range is narrow.The size is uniform and the superficial area is large.Potential of particle surface （Zeta potential）is 27.5 mV.The amorphous calcium phosphate microspheres can well bond with quantum dots.The MTT test results indicate that the ACP microspheres are not toxic to osteoblasts cells （MC3T3-E1）,and have good biocompatibility.

关 键 词：无定形磷酸钙 贻贝贝壳 生物相容性 

分 类 号：Q279[生物学—细胞生物学]