大鼠肝纤维化进程中AT1R/MasR的动态变化  

Dynamic changes of AT1R/MasR in the process of rat liver fibrosis

作　　者：姚乐[1] 李梅 王朝霞[1] 高凤成[1] 毕建红[1] 康凯[1] 安毅[1] YAO Le LI Mei WANG Zhaoxia GAO Fengcheng BI Jianhong KANG Kai AN Yi(The First Hospital of Yulin, Yulin 719000, Shaanxi, China Shaanxi Provincial Tumor Hospital, Xi＇ an 710061, Shaanxi, China)

机构地区：[1]陕西省榆林市第一医院(榆林院区),陕西榆林719000 [2]陕西省肿瘤医院,陕西西安710061

出　　处：《现代中西医结合杂志》2016年第30期3336-3338,3406,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的研究大鼠肝纤维化进程中肝脏AT1R、MasR水平及AT1R/MasR的动态变化。方法将36只雄性Wistar大鼠随机为正常组8只和模型组28只,模型组采用四氯化碳(CCl4)制备大鼠肝纤维化模型,正常组给予腹腔注射等量的生理盐水,分别在干预第2,4,6,8周取各组大鼠肝组织进行HE和Masson三色染色,观察肝组织结构变化情况,并采用Western blot法检测肝组织匀浆中AT1R、MasR蛋白表达情况。结果随着时间延长,模型组大鼠肝纤维化程度逐渐加重;在大鼠肝纤维化进程中,肝组织中AT1R、MasR蛋白表达水平呈上升趋势;第2,4周,模型组AT1R/MasR比值明显低于正常组(P均<0.05),而第6周模型组其比值高于正常组及第4周时(P<0.05),第8周时该比值明显高于第6周时(P<0.05)。结论 AT1R、MasR均参与大鼠肝纤维化形成。Objective It is to investigate the levels of Angiotensin II type 1 receptor（ AT1R） and Angiotensin（ 1- 7） receptor（ MasR）,and the dynamic changes of AT1 R / MasR in the process of rat liver fibrosis. Methods 36 male Winstar rats were randomly divided into model group and normal group,28 rats in the model group were used to prepare rat liver fibrosis model induced by subcutaneous injection of CCl4,and 8 rats in the normal group were treated with intraperitoneal injection of normal saline. 7 rats in the model group and 2 rats in the normal group were killed to get the liver tissue for hematoxylin- eosin（ HE） and Masson trichrome staining after treatment for 2-,4-,6- and 8- week respectively,the changes of liver tissue structure were observed,and the protein expression of AT1 R and MasR in liver homogenate were detected by Western blot analysis. Results The degree of hepatic fibrosis in model group were increased gradually with time. In the process of liver fibrosis,the expression level of AT1 R and MasR in liver tissue was increased. In the model group,the AT1 R / MasR values in the second weeks and the forth weeks were significantly lower than that of the normal group（ all P 〈0. 05）,while those of the model group in the sixth weeks was significantly higher than that in the forth weeks and of the normal group（ all P 〈0. 05）,and in the eighth weeks was significantly higher than that in the sixth weeks（ P 〈0. 05）. Conclusion The occurrence and development of hepatic fibrosis are possibly associated with imbalances between AT1 R and MasR.

关 键 词：肝纤维化 血管紧张素Ⅱ1型受体 血管紧张素(1-7)受体 

分 类 号：R-332[医药卫生]