SLC6A4基因多态性与中国北方人群帕金森病合并抑郁的相关性  

The association between polymorphism of SLC6A4 gene and depression in Parkinson's disease in Chinese Han population

作　　者：刘伟伟[1] 潘旭东[1] 马爱军[1] 张璋[1] 卢克红 

机构地区：[1]青岛大学附属医院神经内科,山东266061

出　　处：《脑与神经疾病杂志》2016年第12期739-743,共5页Journal of Brain and Nervous Diseases

基　　金：青岛市科技计划基础研究项目(11-2-4-2(19)-jch)

摘　　要：目的本文分析SLC6A4基因5-HTTLPR、rs25531位点多态性与中国北方人群帕金森病(PD)合并抑郁的相关性。方法采用病例对照研究方法,选取233例PD患者和252例健康者作为研究对象,应用汉密尔顿抑郁量将PD患者分为抑郁组和非抑郁组;根据患者的运动症状,将PD组分为震颤型、姿势障碍型、不确定型3种亚型;采用限制性片段长度多态性-聚合酶链反应方法(PCR-RELP)和限制性酶切的方法,对SLC6A4基因5-HTTLPR、rs25531位点多态性进行分析。结果与对照组相比,PD组5-HTTLPR、rs25531位点基因型、等位基因型及两位点单倍型分析差异无统计学意义(P>0.05)。与PD非抑郁相比,PD抑郁组5-HTTLPR、rs25531基因型、等位基因型差异无统计学意义(P>0.05);PD患者亚型分析结果显示:5-HTTLPR、rs25531位点在3种亚型中,各分型抑郁组与非抑郁组比较,差异无统计学意义(P>0.05)。结论 SLC6A4基因5-HTTLPR、rs25531位点与PD合并抑郁无关,在PD亚型分析中无差别。Objective To investigate the association between polymorphism of SLC6A4 gene（5-HTTLPR、rs25531 sites） and depression in Parkinson＇s disease in the Han population in north of China. Method We investigated this relationship in a case-control study that comprised 223 PD patients and 252 controls. The PD patients were divided into depression group（123 cases） and the non-depressed group（110 cases） with Hamilton Rating Scale for depression（HAMD）. According to the movement symptoms, the PD group was divided into tremor, postural disorder type, and uncertain three subtypes. We used restriction fragment length polymorphism-polymerase chain reaction（PCR-RELP） and restriction method analyzed the SLC6A4 gene 5-HTTLPR, rs25531 sites polymorphism. Results Compared with the control group, the genotype, allele and haplotype analysis in PD group of 5-HTTLPR, rs25531 locus is not statistically significant（P〈0.05）. Subgroup analysis： the genotype, allele and haplotype analysis of 5-HTTLPR, rs25531 locus is not significant between depression in Parkinson＇s disease and PD patients（P〈0.05）. The analysis of PD patients subtypes showed： there is no significance between depression in patients and non-depressed patients in tremor, postural instability and gait difficulty, and uncertain three subtypes（P〈0.05）. Conclusion Our findings show that there is no relationship between polymorphism of 5-HTTLPR、rs25531 sites and depression in Parkinson＇s disease in the Han population in north of China.

关 键 词：帕金森病 SLC6A4基因 多态性 抑郁 

分 类 号：R742.5[医药卫生—神经病学与精神病学]