358例不育患者Y染色体微缺失分析  

Y chromosome microdeletions analysis of 358 male infertile patients

作　　者：燕凤[1] 徐盈[1] 徐慧[1] 郭芬芬[1] 黎昱[1] 任菊霞[1] 陈必良[1] 张建芳[1] YAN Feng XU Ying XU Hui GUO Fen-fen LI Yi REN Ju-xia CHEN Bi-liang ZHANG Jian-fang.(Department of Gynaecology and Obstetrics, Xijing Hospital, the Fourth Military Medical University, Xi＇ an 710032, China)

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710032

出　　处：《中国优生与遗传杂志》2016年第11期49-50,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究男性不育患者中无精子症和少弱精子症Y染色体微缺失的发生率。方法采用多重PCR技术对358例无精和少弱精患者进行Y染色体AZF微缺失检测。结果 358例无精和少弱精患者中发现AZF微缺失31例,总缺失率为8.7%。无精子症64例,10例Y染色体微缺失,缺失率15.6%;少弱精子症191例,8例缺失,缺失率4.2%;严重少弱精子症103例,13例缺失,缺失率12.6%;31例AZF微缺失中有17例都是AZFc座位序列标签全部缺失+AZFd座位SY152缺失,占缺失总数的55%。结论 Y染色体AZF区域微缺失是引起男性无精子症、少精子症的重要原因之一。AZFc座位序列标签全部缺失+AZFd座位SY152缺失为严重少弱精及无精子症患者Y染色体缺失热区。Objective：To study the incidence of Y chromosome microdeletions in male infertility due to azoospermia and oligozoospermia. Methods：358 azoospermia and oligozoospermia males were selected by multipolymerase chain reaction to detecte of AZF regions of Y chromosome microdeletion. Result：31 cases were found with azoospermia and oligozoospermia males,the proportion was 8.7%. As to the type of microdeletions in AZF and its proportion,64 cases of no sperm patients had 10 cases deletions,191 cases of oligospermia/asthenozoospermia patients had 8 cases deletions,103 cases of severe oligozoospermia or azoospermia patients had 13 cases deletions. The Y chromosome microdeletions was respectively15.6%,4.2%,15.6% in azoospermia,oligoasthenospermia and severe oligozoospermia. Among 31 cases of Y chromosome microdeletions had 17 cases were AZFc＋d regions of SY152 STS deletions,the proportion was 55%. Conclusion：The microdeletions in the AZF region of the Y chromosome is one of the major reasons to cause male infertility. The AZFc＋d regions of SY152 STS deletions was the most frequent in all type of Y chromosome microdeletions.

关 键 词：无精子症 少弱精子症 Y染色体 AZF微缺失 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学]