Susceptibility of northern corn rootworm Diabrotica barberi （Coleoptera： Chrysomelidae） to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins  

作　　者：Isaac O. Oyediran Phillip Matthews Narendra Palekar Wade French Jared Conville Tony Burd 

机构地区：[1]Syngenta Crop Protection, LLC, Research Triangle Park, NC 27709, USA [2]USDA ARS, North Central Agricultural Research Laboratory, Brookings, SD 57006, USA

出　　处：《Insect Science》2016年第6期913-917,共5页昆虫科学（英文版）

摘　　要：The susceptibility of the northern corn rootworm Diabrotica barberi （Smith ＆ Lawrence） to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis （Bt） was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1 Ab proteins were found to be toxic to the northern corn rootworm larvae. The LCs0 and LC99 values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC50 and LC99 values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3, lab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins.The susceptibility of the northern corn rootworm Diabrotica barberi （Smith ＆ Lawrence） to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis （Bt） was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1 Ab proteins were found to be toxic to the northern corn rootworm larvae. The LCs0 and LC99 values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC50 and LC99 values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3, lab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins.

关 键 词：Bacillus thuringiensis Diabrotica barberi eCry3.1Ab mCry3A SUSCEPTIBILITY 

分 类 号：Q789[生物学—分子生物学] S435.132[农业科学—农业昆虫与害虫防治]