整合素α3在髓母细胞瘤干细胞中的表达及对侵袭作用的影响  

Expression of integrin α3 in medulloblastoma stem cells and its influence on invasion effect

作　　者：王勇[1] 王玉社[1] 陈航[1] 梁庆华[1] 陈玉升[1] WANG Yong WANG Yu-she CHEN Hang et al(Department of Neurosurgery, Henan Provincial People＇s Hospital, Zhengzhou 450003, Chin)

机构地区：[1]河南省人民医院神经外科,450003

出　　处：《中国实用医药》2016年第30期27-28,共2页China Practical Medicine

基　　金：河南省医学科技攻关项目(项目编号:201003116);河南省科学技术厅基础研究计划项目(项目编号:142300410072)

摘　　要：目的研究整合素α3在髓母细胞瘤干细胞中的表达及其对侵袭作用的影响。方法应用免疫磁珠法(MACS)从髓母细胞瘤标本中获得CD133+和CD133-细胞,通过免疫组化和实时荧光定量聚合酶链式反应(PCR)检测CD133+和CD133-两组细胞中整合素α3的表达,运用transwell细胞侵袭实验观察使用整合素α3抗体和未使用整合素抗体对CD133+侵袭作用的影响。结果整合素α3在CD133+组的表达显著高于CD133-组(P<0.01);使用整合素α3单克隆抗体组穿膜细胞数目为(26.53±3.71),低于空白对照组的(54.76±6.27)(P<0.01)。结论整合素α3在髓母细胞瘤干细胞的侵袭中起重要作用。Objective To research expression of integrin α3 in medulloblastoma stem cells and its influence on invasion effect. Methods Magnetic activated cell sorting（MACS） was applied to taken CD133＋ and CD133- cells from medulloblastoma samples. Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction（PCR） were used to detect expression of integrin α3 in CD133＋ and CD133- cells. Influence by application of integrin α3 antibody on CD133＋ invasion effect was observed by transwell cell invasion assay. Results Expression of integrin α3 in CD133＋ group was obviously higher than that in CD133-group（P〈0.01）. Monoclonal antibody had lower transmembrane cell number by integrin α3 as（26.53±3.71） than（54.76±6.27） in the blank control group（P〈0.01）. Conclusion Integrin α3 shows important effect in invasion of medulloblastoma stem cells.

关 键 词：髓母细胞瘤 干细胞 整合素Α3 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]