天蚕素B串联体在紫花苜蓿中的表达  

Expression of Cecropin B as Tandem Multimers in Transgenic Alfalfa

作　　者：刘秀明[1,2] 李晓璇[2] 王晶晶[2] 杜淑环[1] 姚娜[1] 孟璐璐[3] 李海燕[1,2] LIU Xiu-ming LI Xiao-xuan WANG Jing-jing DU Shu-huan YAO Na MENG Lu-lu LI Hai-yan(Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development Jilin Agricultural University, Changchun 130118, China College of Life Sciences, Jilin Agricultural University, Changchun 130118, China Science and Technology department of Jilin Province, Changchun 130118, China)

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]吉林省科学技术厅,吉林长春130118

出　　处：《中国草地学报》2016年第6期1-7,共7页Chinese Journal of Grassland

基　　金：国家高技术研究发展计划("863")项目(2011AA100606);国家自然科学基金项目(31101172;31501366);吉林省科技厅项目(20100147;20150623024TC-11);大学生创新创业训练计划项目(201410193039;201510193033)

摘　　要：根据植物偏好性密码子进行改造获得了天蚕素B基因,又通过同尾酶连接获得2个和3个天蚕素B基因的串联体,分别构建植物表达载体,并采用农杆菌介导的方法转化紫花苜蓿。对转基因植物进行分子生物学检测,结果表明CB、CB2、CB3三个基因成功整合到了苜蓿基因组中,转基因植物总蛋白活性检测表明转CB、CB2、CB3基因的蛋白均有一定的抑菌活性,但串联CB3基因的抑菌活性最强。Cecropin B gene was designed and synthesized by plant preference codon,tandem two and three genes（CB2and CB3）were obtained by the tail enzyme.The plant expression vector containing these three genes were constructed respectively.Then,transformed them into alfalfa,by agrobacterium.Integration of the CB multimers gene into the alfalfa genome was confirmed by molecular identification.The total protein of transgenic alfalfa were extracted to detect its bacterial activation,in vivo studies of the protein from the transgenic alfalfa plant demonstrated significant resistance to bacterial wilt and bacterial spot,but bioactivity of the CB-multimers of three genes was higher than bioactivity of single CB.

关 键 词：天蚕素B 抗菌肽 紫花苜蓿 串联表达 抑菌活性 

分 类 号：Q786[生物学—分子生物学]