CHD5和E-cadherin在鳞状细胞喉癌的表达及临床病理意义  

The expression and clinical significance of CHD5 and E-cad proteins in laryngeal squamous carcinoma cells

作　　者：王瑾[1] 赵志国[2] 孙欣[1] 杜国强[1] 

机构地区：[1]中国医科大学附属盛京医院耳鼻喉科,辽宁沈阳110004 [2]中国医科大学附属盛京医院口腔外科,辽宁沈阳110004

出　　处：《解剖科学进展》2016年第6期617-620,624,共5页Progress of Anatomical Sciences

基　　金：国家自然科学青年基金(81202126)

摘　　要：目的检测CHD5与上皮钙粘蛋白(E-cad)在鳞状细胞喉癌(LSCC)组织中的表达,探讨其与分期、淋巴结转移及预后的关系,并分析两者的相关性。方法通过免疫组织化学法和Western印迹法检测103例喉癌患者中,CHD5和E-cadherin蛋白的表达水平,并分析二者对喉癌患者预后的影响。结果 CHD5和E-cad蛋白在喉癌组织表达水平显著低于癌旁正常组织(P<0.05),二者的表达水平与LSCC患者的TNM分期、淋巴结转移及预后呈显著负相关,而与患者的性别、年龄因素无相关性。CHD5蛋白与E-cad蛋白的表达的平均光密度值(AOD)呈显著正相关(r=0.814,P<0.01)。结论 CHD5基因可能通过调控E-cad蛋白的表达参与了喉癌上皮间质转化的过程。Objective To investigate the expression of CHD5 and E-cad proteins in laryngeal squamous cell carcinoma（LSCC） and the clinicopathological significance. Methods The expression of CHD5 protein and E-cad protein were detected with immunohistochemical SP technique and Western bloting in 103 cases of laryngeal squamous cell carcinoma. Results The expression levels of CHD5 protein and E-cad protein were down regulated in 103 LSCC tissue than in normal tissue（P〈0.05）, and negatively correlated to TNM staging and lymph node metastasis and prognosis of LSCC, but not related to sex and age. The expression levels of CHD5 and E-cad protein in 103 LSCC tissue were correlated positively（r=-0.814, P〈0.01）. Conclusion CHD5 gene might participate in progress of epithelial-mesenchymal transition（EMT） of LSCC via regulating E-cad expression.

关 键 词：CHD5 E-CADHERIN 鳞状细胞喉癌 

分 类 号：R739.65[医药卫生—肿瘤]