miR-145在胶质瘤中的表达及其对胶质瘤增殖及侵袭的影响  

Expression of miR-145 in brain glioma and its role in proliferation and invasion of U87 glioma cells

作　　者：曹英海[1] 夏春义[1] 王刚[1] 李纳新[1] 

机构地区：[1]沈阳市第一人民医院神经外科,辽宁沈阳110041

出　　处：《解剖科学进展》2016年第6期633-636,共4页Progress of Anatomical Sciences

基　　金：辽宁省科技攻关资助项目(201225020)

摘　　要：目的探讨miR-145在胶质瘤中的表达及其对胶质瘤增殖侵袭的影响。方法用实时荧光定量PCR方法检测39例胶质瘤组织及其对应癌旁组织miR-145的表达;利用LipofectA MINET M2000将miR-145模拟物或抑制剂及其阴性对照转染至人U87胶质瘤细胞,分别应用CCK-8法和Transwell小室法检测人胶质瘤U87细胞的增殖及侵袭能力。结果胶质瘤组织中miR-145的相对表达量为17.26±6.05,癌旁组织的miR-145相对表达量为53.58±21.26,胶质瘤组织中miR-145的表达显著低于癌旁组织(P<0.01)。与对照组相比,miR-145模拟物能够显著抑制胶质瘤U87细胞系的增殖以及侵袭能力(P<0.01);miR-145抑制剂则能够显著上调胶质瘤U87细胞系的增殖以及侵袭能力(P<0.01)。结论miR-145在胶质瘤组织中是低表达的,在胶质瘤U87细胞中过表达miR-145能够抑制细胞的增殖及侵袭能力。Objective To study the expression of miR-145 in brain glioma and its role in proliferation and invasion of U87 glinma cells. Methods miR-145 expression was detected by quantitative qRT-PCR in brain glioma tissues and paraneoplastic tissues of 39 patients. LipofectAMINETM2000 was used to transfect miR-145 mimics, inhibitors and its negative control into human U87 glioma cells, and then CCK-8 assay and Transwell chamber assay were applied to measure the proliferation, migration and invasion of human U87 glioma cells. Results miR-145 expression level was lower in brain glioma tissues（17.26 ± 6.05） than in paraneoplastic tissues（53.58± 21.26, P〈 0.01）. Compared with control group, miR-145 mimics could significantly inhibit the abilities of proliferation, migration and invasion of human glioma U87 cells, and miR-145 inhibitors could significantly upregulate the abilities of proliferation, migration and invasion of human glioma U87 cells. Conclusion miR-145 lowexpressed in brain glioma tissues, miR-145 overexpression was involved in the inhibition of proliferation and invasion of U87 glioma cells.

关 键 词：U87胶质瘤细胞 MIR-145 增殖 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]