自动尿液细胞DNA定量分析对泌尿系统炎症与膀胱癌的鉴别诊断价值  

The value of automated urine cell DNA quantitative analysis in the differential diagnosis of urinary tract inflammation and bladder cancer

作　　者：吴康[1] 满艳茹[1] 唐文潇 俞靖龙[1] 周道银[1] 邓安梅[1] 孙懿[1] 颜宏利[1] 

机构地区：[1]第二军医大学长海医院实验诊断科,上海200433

出　　处：《中国临床医学》2016年第5期629-632,共4页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金(81302579;81471605);上海申康医院发展中心市级医院临床辅助科室能力建设项目基金(SHDC22014014)~~

摘　　要：目的:评估尿液细胞DNA定量分析在泌尿系统肿瘤诊断中的应用价值。方法:采集92例泌尿系统炎症和39例疑似膀胱癌患者的尿液分别进行DNA定量测定、常规细胞学检验和尿液液基细胞学检查,以病理诊断为金标准,评估3种方法对泌尿系统炎症与膀胱癌的鉴别诊断价值,分析尿液细胞DNA定量与肿瘤分型的关联。结果:尿液DNA倍体分析对膀胱癌诊断的敏感性和特异性分别为88.89%和97.09%,诊断符合率为94.66%。传统尿液细胞学检验的敏感性和特异性分别为51.85%和100%,准确性为90.07%。尿液液基细胞学检查对膀胱癌诊断的敏感性和特异性分别为81.48%和99.04%,诊断符合率为95.41%。尿液DNA倍体分析结果>5C细胞的个数与尿路上皮癌分型存在关联,高级别尿路上皮癌组异倍体(>5C)细胞数量较低级别组显著增高。结论:尿液DNA定量分析能够较好地鉴别泌尿系统炎症与膀胱癌,较传统细胞学检验有更好的提示作用。Objective：To evaluate the application value of urine cell DNA quantitative analysis in the diagnosis of urinary tract tumors.Methods：There were 92 cases of urinary tract inflammation and 39 cases of patients with suspected bladder cancer urine were tested by DNA quantitative determination,conventional cytology test and urine cytology,pathological diagnosis as the gold standard,the value of the three methods in differential diagnosis of urinary tract inflammation and bladder cancer were assessed,the correlationship between urine cell DNA quantitative and tumor types was analyzed.Results：The sensitivity and specificity of urinary DNA ploidy analysis for the diagnosis of bladder cancer were 88.89% and97.09%,respectively,and the diagnostic accuracy was 94.66%.The sensitivity and specificity of the traditional urine cytology test were 51.85% and 100%,respectively,and the accuracy was 90.07%.The sensitivity and specificity of urine liquid based cytology test in the diagnosis of bladder cancer were 81.48% and 99.04%,respectively,and the diagnostic accuracy was95.41%.Urine DNA ploidy analysis results〉 5C cell number and the type of the urinary tract epithelial cancer were associated,high level of urinary tract epithelial cancer group（〉5C）cell number was significantly higher than the low level group.Conclusions：The urine DNA quantitative analysis can identify the urinary system inflammation and bladder cancer,which is better than the traditional cytology test.

关 键 词：自动尿液细胞DNA定量分析 泌尿系统炎症 膀胱癌 

分 类 号：R737.1[医药卫生—肿瘤]