人乳头瘤病毒基因亚型检测在子宫颈疾病诊断中的意义  

Significance of HPV genotype detection in diagnosis of cervical lesion

作　　者：顾峥嵘[1] 吴红[1] 王玲[1] 

机构地区：[1]同济大学附属第一妇婴保健院妇科,上海200040

出　　处：《诊断学理论与实践》2016年第5期517-520,共4页Journal of Diagnostics Concepts & Practice

摘　　要：目的:探讨不同人乳头瘤病毒(human papillomavirus,HPV)基因亚型的感染情况及高危型HPV亚型感染与子宫颈疾病间的关系。方法:选取我院妇科门诊就诊的1 669例患者行HPV基因分型检测,HPV检测结果为阳性的患者在知情同意后行阴道镜检查,对镜下表现可疑的患者进一步行子宫颈组织活检。结果:1 669例患者中HPV阳性567例,总HPV阳性率为33.97%,HPV亚型感染率前5位由高到低依次为HPV-16(9.05%)、HPV-52(7.85%)、HPV-58(6.29%)、HPV-CP8304(5.81%)、HPV-53(3.71%)。不同年龄段中,≤30岁者为HPV感染的高峰年龄段。在567例HPV阳性的患者中,高危型HPV亚型感染患者共454例,占总HPV感染者的80.07%。病灶组织活检结果为高级别鳞状上皮内病变(high-grade squamous intraepithelial lesion,HSIL)以上的患者中,高危型HPV亚型感染的前5位由高到低依次为HPV-16(58例)、HPV-52(26例)、HPV-58(25例)、HPV-18(10例)、HPV-31(6例)。结论 :HPV感染人群高峰有年轻化趋势,HPV基因分型检测在子宫颈疾病诊断中具有重要价值,其中HPV-16、HPV-52、HPV-58、HPV-18、HPV-31与子宫颈癌的关系密切。Objective： To investigate the infection of different genotypes of papillomavirus（HPV） and the relationship between high-risk HPV genotype and cervical lesion in females. Methods： HPV genotype detection was performed in 1 669 subjects. Colposcopy was conducted in positive cases after informed consent was obtained and biopsy was performed in suspected cases under colposcopy. Results： Five hundred and sixty-seven cases of the 1 669 subjects were HPV positive, with a positive rate of 33.97%（567/1 669）. The five leading frequencies of HPV genotype were HPV-16（9.05%）, HPV-52（7.85%）, HPV-58（6.29%）, HPV-CP8304（5.81%）, HPV-53（3.71%）. Women ≤30 were at the peak of HPV infection when compared with other ages. Four hundred and fifty-four cases of 567 HPV positive subjects were infected with high-risk HPV genotype. The five leading frequencies of high-risk HPV infection with high grade squamous intraepithelial lesion（HSIL） in biopsy were HPV-16（58 cases）, HPV-52（26 cases）, HPV-58（25 cases）, HPV-18（25 cases）, HPV-31（6 cases）. Conclusions： The peak age for HPV infection is getting younger, and HPV genotype detection has significant value in the diagnosis of cervical lesion, especially HPV-16, HPV-52, HPV-58, HPV-18, HPV-31,they are closely associated with cervical cancer.

关 键 词：人乳头瘤病毒 基因分型 宫颈疾病 

分 类 号：R737.33[医药卫生—肿瘤]