Sporamin对人胰腺癌细胞增殖、迁移和侵袭以及Notch4蛋白表达的影响  

Effect of sporamin on expression of Notch4 protein in human pancreatic cancer cells and proliferation,migration,invasion of the tumor cells

作　　者：陈依依[1] 曾菊萍 陈晓英[1] 刘富强[1] 姚军[1] 钱翠娟[2] 

机构地区：[1]台州学院医学院医学检验教研室,浙江台州318000 [2]台州学院医学院临床医学教研室,浙江台州318000

出　　处：《中国肿瘤生物治疗杂志》2016年第6期779-782,共4页Chinese Journal of Cancer Biotherapy

基　　金：浙江省自然科学基金资助项目(No.LY16H160033);浙江省公益技术应用研究项目(No.2016C33189)~~

摘　　要：目的:探讨胰蛋白酶抑制剂sporamin对人胰腺癌细胞增殖、迁移和侵袭及Notch4蛋白表达的影响。方法:采用MTT法检测sporamin对人胰腺癌细胞株PANC-1和MiaPaCa-2增殖能力的影响,Transwell法检测胰腺癌细胞体外迁移和侵袭能力,Western blotting检测不同质量浓度的sporamin(0、25、50、75、100μg/ml)对人胰腺癌细胞中Notch4蛋白表达水平的影响。结果:Sporamin能显著抑制人胰腺癌PANC-1和MiaPaCa-2细胞的增殖、迁移和侵袭能力,三种作用均呈显著质量浓度依赖性(P<0.05),但无显著时间依赖性(P>0.05);随着sporamin质量浓度的增加,Notch4蛋白水平的表达也逐渐下降。结论:胰蛋白酶抑制剂sporamin能质量浓度依赖性抑制人胰腺癌细胞增殖、迁移和侵袭能力,同时抑制人胰腺癌细胞中Notch4蛋白的表达。Objective： To investigate effect of trypsin inhibitor, sporamin, on expression of Notch4 protein in human pancreatic cancer （PC） cells and proliferation, migration, invasion of the tumor cells. Methods： Effect of the sporamin on proliferation of the PANC-I and MiaPaCa-2 PC line ceils was detected by MTI＇ assay ; proliferation and invasion abili- ties in vitro of the pancreatic cancer cells detected by Transwell assay and effects of various concentrations of the sporamin （0,25,50,75 and 100 μg/ml） on expression level of the Notch4 protein in the human pancreatic cancer cells detected by Western blotting assay. Results： Sporamin significantly inhibited prolilferation, migration and invasion abilities of the PANC-I and MiaPaCa-2 PC line cells, as a patern of concentration-dependence （P 〈 0.05 ）, but not a patern of time-de- pendence （ P 〉 0.05 ）. With increase of the sporamin concentration, expression level of the Notch4 protein also decreased gradually. Conclusion： Trypsin inhibitor sporamin could inhibit proliferation, migration and invasion abilities of the PC cells with a concentration-dependence, and simualtaneously inhibit expression of the Notch4 protein in the PC cells.

关 键 词：胰蛋白酶抑制剂 SPORAMIN 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]