鳕鱼皮寡肽对人胃癌细胞BGC-823增殖影响的实验研究  

Effect of cod skin oligopeptide on proliferation of human gastric carcinoma BGC-823 cells

作　　者：张国强 胡晓斐 续力云 吴丽萍 

机构地区：[1]舟山医院胃肠外科,316021

出　　处：《浙江医学》2016年第23期1864-1867,1875,共5页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目(LY12C20002);浙江省科技厅公益类项目(2011C23034)

摘　　要：目的探讨鳕鱼皮寡肽(cod skin of oligopeptide,CSO)对人胃癌细胞(BGC-823)增殖的影响。方法用不同浓度(20、40、60、80mg/ml)CSO处理体外培养的BGC-823,72h后显微镜下观察细胞生长情况;分别于24、48、72h,应用CCK-8法检测细胞增殖情况并计算50%细胞抑制率(IC_(50));处理后24h采用流式细胞术测定细胞凋亡及细胞周期的改变情况。结果与对照组相比,经CSO处理后,镜下BGC-823数目减少。CCK-8检测结果显示,BGC-823数目减少呈剂量、时间依懒性(均P<0.05),并且CSO对BGC-823作用24、48和72h的IC_(50)分别是87.34、68.14、44.55mg/ml。对照组BGC-823存活率为(86.62±10.32)%,60mg/ml组BGC-823存活率为(24.18±6.59)%,差异有统计学意义(P<0.05);对照组BGC-823晚期凋亡率为(1.81±0.35)%,60mg/ml组BGC-823晚期凋亡率为(55.84±5.89)%,差异有统计学意义(P<0.05)。在G1期,对照组BGC-823细胞分布为(61.13±10.32)%,60mg/ml组BGC-823细胞分布为(79.74±10.21)%,差异有统计学意义(P<0.05);在S期,对照组BGC-823细胞分布为(23.04±8.64)%,60mg/ml组BGC-823细胞分布为(10.42±5.36)%,差异有统计学意义(P<0.05);在G2期,20mg/ml组BGC-823细胞分布为(9.96±2.68)%,40mg/ml组BGC-823细胞分布为(6.93±1.25)%,60mg/ml组BGC-823细胞分布为(9.84±3.79)%,与对照组BGC-823细胞分布(15.83±5.89)%相比,差异均有统计学意义(均P<0.05)。结论 CSO可阻滞BGC-823生长周期,促进BGC-823凋亡,抑制增殖,从而抑制肿瘤细胞的生长。Objective To explore the effect of cod skin oligopeptide（CSO） on the proliferation of human gastric carcinoma BGC-823 cells. Methods BGC-823 cells were treated with different concentrations（20, 40, 60, 80mg/m L） of CSO. Cell morphology was observed under the microscope, cell viability was determined by CCK-8 assay, and ell apoptosis and cell cycle were detected by flow cytometry. Results CSO significantly suppressed the proliferation of BGC-823 cells in a time- and dose-dependent manner. IC50 of CSO with 24 h, 48 h and 72 h were 87.34mg/ml, 68.14mg/ml and 44.55mg/ml, respectively. The early apoptosis rate of BGC-823 cells was（17.55±5.48）% and the late apoptosis rate was（55.84±5.89）%, when the cells were treated with 80mg/ml CSO. After BGC-823 cells were treated with CSO for 24 h, the cell cycle arrested at S/G2 stage. The number of G1 cells increased with the increasing of CSO concentration, peaked at（79.74±10.21）% when CSO concentration was 60mg/ml,the number of S/G2 cells were decreased with the increasing of CSO concentration, reached the lowest value of（9.84±3.79）%when CSO was 60mg/ml. Conclusion CSO can inhibit the proliferation and promote the apoptosis of human gastric cancer BGC-823 cells.

关 键 词：鳕鱼皮寡肽 人胃癌细胞 BGC-823 细胞周期 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]