WTAP基因在胃癌组织中低表达  

Low expression of WTAP gene in gastric cancer

作　　者：汪毅[1] 张杰[2] 皮静楠 刘晓玲[2] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤医院,北京100021 [2]中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2016年第11期1548-1553,共6页Basic and Clinical Medicine

摘　　要：目的探讨WTAP基因在胃癌组织样本中的表达变化情况,并检测其对胃癌细胞表型的影响。方法用RTq PCR的方法检测45例胃癌组织及其对应的癌旁组织中WTAP mRNA的表达量,并结合临床病理资料进行分析;使用RNA干扰方法敲低内源性WTAP在胃癌细胞系MGC-803中的表达,再通过CCK-8实验、划痕迁移实验和transwell实验,检测WTAP表达降低后对MGC-803细胞功能的影响。结果 WTAP mRNA在胃癌癌症组织中的表达明显低于癌旁组织(P<0.05),且其表达与胃癌样本的临床分期密切相关(P<0.05);在MGC-803细胞系中敲低WTAP,可促进胃癌细胞增殖、促进细胞迁移和侵袭。结论 WTAP在胃癌组织中低表达,可能与其发生发展有关,有望成为胃癌诊治的靶标。Objective To investigate the expression change of WTAP gene in gastric cancer samples and its functional significance in gastric carcinogenesis. Methods The WTAP mRNA was detected by qRT-PCR in 45 cases of gastric cancer samples and their paired adjacent normal control tissues. The expression of WTAP mRNA was subjected to analysis combined with patients＇ clinical pathological data. The effects of WTAP knock-down on cell proliferation,cell migration and invasion were detected by CCK-8,wounding heal and trans-well assays,respectively. Results The expression of WTAP mRNA in gastric tumor tissues was significantly lower than that of adjacent tissues（ P〈0. 05）,and was closely related with the cancer stage（ P〈0. 05）. Furthermore,knock-down of WTAP in MGC-803 cells promoted cell proliferation,cell migration and invasion. Conclusions WTAP is down-regulated in gastric cancers,and it may serve as a tumor suppressor gene in gastric carcinogenesis.

关 键 词：胃癌 WTAP MGC-803细胞 

分 类 号：R735.2[医药卫生—肿瘤]