响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性  

Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Derris eriocarpa Based on Response Surface Method

作　　者：杨立芳[1] 刘洪存 支媛[1] 郭洋洋[1] 闫文超[2] 姜明国[3] 谭学才[1] 石灵芳 

机构地区：[1]广西民族大学化学化工学院广西高校微生物与植物资源利用重点实验室广西高校食品安全与药物分析化学重点实验室,南宁530006 [2]中国药科大学药学院,南京210009 [3]广西民族大学海洋与生物技术学院,南宁530006

出　　处：《中国实验方剂学杂志》2016年第23期28-34,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81560713);广西教育厅科学研究项目(YB2014101)

摘　　要：目的:利用响应曲面分析法优化毛果鱼藤总生物碱的提取工艺,并检测其抑菌活性。方法:以毛果鱼藤为原料,采用微波提取法,设计单因素实验,分别考察了料液比、乙醇体积分数、提取时间、微波提取功率、提取次数5个单因素对毛果鱼藤总生物碱提取效果的影响;采用响应曲面法优化毛果鱼藤总生物碱的提取工艺,提高总生物碱的提取效率,并考察总生物碱的体外抑菌作用。结果:毛果鱼藤总生物碱的最佳提取工艺条件为微波功率400 W,提取温度为70℃,料液比为1∶23,乙醇体积分数为70%,提取时间为23 min,在此条件下,以盐酸小檗碱计,总生物碱提取率为0.107 7 mg·g-1;抑菌实验表明,毛果鱼藤总生物碱对金黄色葡萄球菌有一定的抑菌作用。结论:微波法提取生物碱工艺方法科学、稳定,且生物碱具有良好的抑菌作用。Objective： To optimize the extraction conditions of total alkaloids from Derris eriocarpa by response surface method,and detect their antibacterial activity. Method： Microwave extraction was used for onesingle factor experiment of D. eriocarpa to investigate the effects of solid-liquid ratio,ethanol volume fraction,extraction time,microwave power,and number of extraction times on the extraction of total alkaloids from D. eriocarpa. Response surface method（ RSM） was employed to optimize the extraction process,improve the extraction efficiency of total alkaloids,and investigate the in vitro antibacterial effect of total alkaloids. Result： The optimal extraction parameters were as follows： microwave extraction power of 400 W,extraction temperature of 70℃,solid-liquid ratio of 1 ∶ 23,extraction time of 23 min and ethanol volume fraction of 70%. The experiment extraction yield of the total alkaloids was 0. 107 7 mg·g-1under these optimal conditions measured by berberine hydrochloride. The antibacterial activity data suggested that the total alkaloids had a certain inhibitory effect on the Staphylococcus aureus. Conclusion： The microwave extraction method for total alkaloids from D. eriocarpa was efficient and stable,and the total alkaloids showed good antibacterial effect.

关 键 词：毛果鱼藤 总生物碱 抑菌活性 响应曲面 

分 类 号：R282.2[医药卫生—中药学] R283.6[医药卫生—中医学]