芫花根的化学成分  

Chemical Constituents from Roots of Daphne genkwa

作　　者：孙文婷[1] 蔡由生[1] 杨升平[1] 

机构地区：[1]武汉大学药学院,武汉430071

出　　处：《中国实验方剂学杂志》2016年第23期49-54,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：武汉大学引进人才科研启动项目(306-410100004)

摘　　要：目的：研究芫花Daphne genkwa根的化学成分,并筛选其生物活性。方法：采用硅胶柱色谱,凝胶柱色谱等手段,结合理化性质和波谱数据,对芫花根的乙酸乙酯萃取部分的化合物进行分离鉴定。结果：从芫花根中分离得到了16个化合物,分别鉴定为二氢瑞香素乙（dihydrodaphnodorin B,1）,荛花醇A（wikstrol A,2）,瑞香酚（stelleranol,3）,毛瑞香素D2（daphnodorin D2,4）,毛瑞香素D1（daphnodorin D1,5）,毛瑞香素A（daphnodorin A,6）,O-methyledgeworin（7）,clemaphenol A（8）,1β-hydroxy-10βH-guaia-4,11-dien-3-one（9）,1αH,10αH,7αH-guaia-4,11-dien-3-one（10）,1,5-diphenylpentan-1-hydroxy-3-one（11）,oleodaphnone（12）,S-（＋）-1,5-diphenylpentan-3-hydroxy-1-one（13）,8-hydroxypluviatolide（14）,表木栓醇（epifriedelanol,15）,5α-stigmast-9（11）-en-3β-ol（16）。生物活性筛选结果表明,化合物1,2,6对金黄色葡萄球菌有抑制作用。结论：化合物1-6,10,12,13首次从芫花中分离得到,化合物11,15首次从瑞香属中分离得到,化合物7-9,14,16首次从瑞香科中分离得到。Objective： To study the chemical constituents in the roots of Daphne genkwa,and screen their biological activity. Method： The compounds were isolated by silica gel column chromatography,Sephandex LH-20,and their structures were elucidated by means of spectral analysis. Result： Sixteen compounds were isolated and identified as dihydrodaphnodorin B（ 1）,wikstrol A（ 2）,stelleranol（ 3）,daphnodorin D2（ 4）,daphnodorin D1（ 5）,daphnodorin A（ 6）,O-methyledgeworin（ 7）,clemaphenol A（ 8）,1β-hydroxy-10βHguaia-4,11-dien-3-one（ 9）, 1αH, 10αH, 7αH-guaia-4, 11-dien-3-one（ 10）, 1, 5-diphenylpentan-1-hydroxy-3-one（ 11）, oleodaphnone（ 12）, S-（ ＋）-1, 5-diphenylpentan-3-hydroxy-1-one（ 13）, 8-hydroxypluviatolide（ 14）,epifriedelanol（ 15）,and 5α-stigmast-9（ 11）-en-3β-ol（ 16）. The screening results of biological activity showed that compounds 1,2,6 had inhibitory effect on Staphylococcus aureus. Conclusion：Compounds 1-6,10,12,13 were isolated from this plant for the first time. Compounds 11,15 were isolated from the plants of this genus for the first time. Compounds 7-9,14,16 were first obtained from the plants of Thymelaeaceae.

关 键 词：芫花 化学成分 双黄酮 抑菌活性 二氢瑞香素乙 荛花醇A 毛瑞香素A 

分 类 号：R284.1[医药卫生—中药学]