抗肿瘤药物纳米胶束制剂对人肝癌干细胞作用的研究  

Effect of epirubicin nanoparticles on liver cancer stem cells

作　　者：肖丽丽[1] 梁暖 徐晖[3] 

机构地区：[1]大庆油田总医院消化内科,黑龙江大庆163000 [2]复旦大学附属中山医院青浦分院内镜室,上海200032 [3]上海中医药大学附属第七人民医院消化内科,上海200000

出　　处：《哈尔滨医科大学学报》2016年第5期434-436,共3页Journal of Harbin Medical University

摘　　要：目的探讨抗肿瘤药物纳米胶束对肝癌干细胞的作用。方法培养Hep G2人肝癌细胞株,利用OCT-4的抗体对所获细胞样品进行免疫分拣,逆转录PCR检测OCT-4 mRNA水平,确认所培养的细胞高表达OCT-4,获得高表达OCT-4的肝癌干细胞。制备表阿霉素纳米胶束。实验分组为表阿霉素组、表阿霉素纳米胶束组及空白对照组。用药后采用原位末端标记法检测肿瘤组织细胞凋亡;利用免疫组化检测核增殖抗原(PCNA)的表达。结果获得了高表达OCT-4的肝癌干细胞株;制备表阿霉素纳米胶束,分散性好。表阿霉素纳米胶束组能诱导细胞凋亡,与未用药对照组及表阿霉素组相比均差异显著(P<0.05);表阿霉素纳米胶束组能抑制癌细胞增值,降低PCNA的表达,与未用药对照组、表阿霉素组相比均差异有统计学意义(P<0.05)。结论表阿霉素纳米胶束能诱导细胞凋亡,并能降低PCNA的表达。Objective To study the method of preparation of epirubicin nanoparticles and its effects on liver cancer model. Methods Human liver cancer cell line HepG2 were cultured. Immune cells were sorted by use of OCT-4 antibody. Liver cancer stem cells with high expression of OCT-4 were obtained by reverse transcription PCR. Epirubicin nanoparticles were prepared by sol-gel method. Cells were divided into 3 groups： control group （ no treatment）, epirubicin nanoparticles group, epirubicin group. After treated, TUNEL were performed to examine apoptosis. The expression of PCNA was detected by immunohistochemistry. Results Liver cancer stem cells with high expression of OCT-4 were obtained. Epirubicin nanoparticles prepared were well dispersive. The epirubicin nanopaticles produced a stronger apoptotis effect on tumor cells than epirubicin group and control group （ P 〈 0. 05 ）, as well as depressd expression of PCNA. Conclusion Epirubicin nanopaticles can induce the apoptosis of tumor, inhibit the expression of PCNA.

关 键 词：肝癌干细胞 表阿霉素 纳米胶束 PCNA 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]