慢性粒细胞白血病患者骨髓部分细胞因子的表达及意义  

Expression of partial cytokines in bone marrow of chronic myeloid leukemia patients and its significance

作　　者：宋建新[1] 张芹[1] 梅芬[2] 欧阳红梅[1] 蒋雅先[1] 撒亚莲[2] 

机构地区：[1]云南省第一人民医院/昆明理工大学附属医院血液病诊断室,昆明650032 [2]云南省第一人民医院/昆明理工大学附属医院临床基础研究所,昆明650032

出　　处：《国际检验医学杂志》2016年第23期3255-3257,共3页International Journal of Laboratory Medicine

基　　金：云南省科技厅-昆明医科大学联合专项应用基础研究项目资助(2014FZ070)

摘　　要：目的探讨慢性粒细胞白血病(CML)患者不同时期骨髓白细胞介素(IL)-1β、IL-2、IL-4、IL-6、IL-10及干扰素-γ(INF-γ)表达的变化。方法应用流式细胞仪分析30例CML慢性期(CML-CP)、21例加速期(CML-AP)、15例急变期(CMLBP),经治疗后缓解患者42例、未缓解患者7例的骨髓液中IL-1β、IL-2、IL-4、IL-6、IL-10及INF-γ表达水平,并与对照组比较。结果CML各组患者INF-γ、IL-2水平均低于对照组(P<0.05),IL-1β、IL-4、IL-6、IL-10均高于对照组(P<0.05)。随着病情进展,INF-γ、IL-2水平逐渐降低,即CML-BP<CML-AP<CML-CP,且组间比较差异有统计学意义(P<0.05);IL-1β、IL-4、IL-6、IL-10逐渐增高,即CML-BP>CML-AP>CML-CP,组间比较差异有统计学意义(P<0.05)。患者经治疗完全缓解后,INF-γ、IL-2有所回升,但仍低于对照组(P<0.01),IL-1β、IL-4、IL-6、IL-10有所降低,同样也高于CML-CP(P<0.05);未缓解组IL-1β、IL-4、IL-6、IL-10及IL-2、INF-γ与急变期相比差异无统计学意义(P>0.05)。结论在CML患者骨髓微环境中细胞因子水平的变化对CML的发生、发展及与预后评估有一定的意义;检测CML骨髓IL-1β、IL-2、IL-6、IL-10及INF-γ水平有望为CML患者免疫治疗及预后判断提供新的思路和理论依据。Objective To investigate the changes of IL-1β,IL-2,IL-4,IL-6,IL-10 and INF-γexpressions in bone marrow of chronic myeloid leukemia（CML）patients.Methods The IL-1β,IL-2,IL-4,IL-6,IL-10 and INF-γexpression levels were detected by using flow cytometry in 30 cases of CML chronic phase（CML-CP）,21 cases of CML accelerated phase（CML-AP）,15 cases of CML blastic phase（CML-BP）,42 cases of CML remission after treatment and 7cases of non-remission.Then the detection results were compared with those in the control group.Results The expression levels of INF-γand IL-2in each CML groups were lower than those in the control group（P〈0.05）,while the expression levels of IL-1β,IL-4,IL-6and IL-10 were higher than those in the control group（P〈0.05）.With the disease condition progression,the INF-γand IL-2levels were gradually decreased,i.e.,CML-BPCML-APCML-CP,moreover which had statistical difference among groups（P〈0.05）;the expression levels of IL-1β,IL-4,IL-6and IL-10 were gradually increased,i.e.,CML-BPCML-APCML-CP,the difference among groups had statistical significance（P〈0.05）.After complete remission by treatment,the INF-γand IL-2levels were risen to some extent,but which were still lower than those in the control group（P〈0.01）,the expression levels of IL-1β,IL-4,IL-6and IL-10 were decreased to some extent,but likewise were higher than those in CML-CP（P〈0.05）;the levels of IL-1β,IL-4,IL-6,IL-10,IL-2and INF-γhad no statistical difference between the non-remission group and CML-BP group（P〈0.05）.Conclusion The changes of serum cytokines in bone marrow microenvironment of CMLpatients have certain significance to the occurrence,development and prognosis of CML;the detection of IL-1β,IL-2,IL-4,IL-6,IL-10 and INF-γlevels in bone marrow is hopeful to provide new ideas and theoretical basis for immune therapy and prognosis judgment of CML patients.

关 键 词：慢性粒细胞白血病 白细胞介素 干扰素-Γ 

分 类 号：R733.7[医药卫生—肿瘤] R730.43[医药卫生—临床医学]