精子DNA冷冻损伤易感性与精子活动力相关性  

The association between susceptibility of sperm DNA damage to freezing and sperm motility

作　　者：吴永根[1] 郑九嘉[1] 胡黎川 杨旭[1] 张李雅[1] 黄学锋[1] 金建远[1] WU Yong-gen ZHENG Jiu-jia HU Li-chuan YANG Xu ZHANG Li-ya HUANG Xue-feng JIN Jian-yuan(Reproductive Medicine Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000 Department of Pharmacy ,Wenzhou Central Hospital ,Wenzhou 325000)

机构地区：[1]温州医科大学附属第一医院生殖医学中心,温州325000 [2]温州市中心医院药剂科,温州325000

出　　处：《生殖医学杂志》2016年第12期1095-1099,共5页Journal of Reproductive Medicine

基　　金：浙江省卫计委基金(2014KYB341);温州市科技计划项目(Y20130035)

摘　　要：目的研究不同活动力精子其核DNA对冷冻损伤的易感性。方法选取来本中心行精液常规分析,精子浓度>5×106/ml、精子正常形态率>4%者63例为研究对象。精子冷冻前后均采用染色质扩散(SCD)实验分析核DNA完整性,分别比较高活力组(d级<30%,n=28)及低活力组(d级≥30%,n=35)精子在冷冻前后DNA完整性的差异。结果精液标本(n=63)经冷冻后,精子DNA碎片指数(SDF)较冷冻前显著升高[(23.1±12.5)%vs.(19.9±11.6)%,P<0.05],其中代表精子DNA完整性较好的大晕轮精子比例显著降低[(71.9±13.3)%vs.(75.8±12.2)%,P<0.05],而小晕轮精子比例则显著升高[(10.2±5.7)%vs.(8.2±3.9)%,P<0.05]。在低活力组,精子SDF及大晕轮、小晕轮精子比例在冷冻前后亦差异显著(P<0.05);而在高活力组,冷冻后仅小晕轮精子比例较冷冻前显著升高[(8.3±3.4)%vs.(6.8±3.0)%,P<0.05],SDF及大晕轮、中晕轮、无晕轮的精子比例在冷冻前后均无显著差异(P>0.05)。结论相比高活力组精子,低活力组精子核DNA冷冻耐受力较差,更易受损。Objective： To study the susceptibility of sperm DNA damage to the freezing in the spermatozoa with different motility.Methods： Sixty three semen samples with density〉5×106/ml and normal morphology rate〉4%were selected.The sperm DNA integrity was analyzed by the sperm chromatin dispersion（SCD）method.Sperm DNA integrity were compared before and after frozen in the spermatozoa with higher motility（D degree〈30%）or lower motility（D degree≥30%）.Results： The sperm DNA fragmentation（SDF）index was significantly increased after frozen in 63 sperm samples[（23.1±12.5）% vs.（19.9±11.6）%,P〈0.05].In which,the ratio of sperm with big halo,which represents good sperm DNA integrity,was significantly reduced[（71.9±13.3）% vs.（75.8±12.2）%,P〈0.05];the ratio of sperm with small halo was significantly increased [（10.2±5.7）% vs.（8.2±3.9）%,P〈0.05]after frozen.In the sperm with lower sperm motility（D degree≥30%,n=35）,the SDF index,the ratio of sperm with big halo or small halo were significantly different before and after frozen（P〈0.05）.In the sperm with higher motility（D degree〈30%,n=28）,only the ratio of sperm with small halo was significantly increased after frozen[（8.3±3.4）% vs.（6.8±3.0）%,P〈0.05）],the SDF index and the ratio of sperm with big halo or medium halo were not significantly different（P〉0.05）.Conclusions： DNA damage of spermatozoa with lower motility is more susceptible to freezing compared sperm with the high motility.

关 键 词：精子活力 精子DNA碎片指数 男性不育 精子冷冻 

分 类 号：R[医药卫生]