相对和绝对定量同位素标记技术在肝细胞癌蛋白质组学中的应用研究进展  

Research advances in application of isobaric tags for relative and absolute quantitation in proteomics of hepatocellular carcinoma

作　　者：齐英姿 陈凌声[1,2] 徐平[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所北京蛋白质组研究中心蛋白质组学国家重点实验室,北京100850 [2]广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁530005

出　　处：《中华肝脏病杂志》2016年第12期952-955,共4页Chinese Journal of Hepatology

基　　金：国家重点基础研究发展计划（2011CB910600,2013CB911200）;国家高技术研究发展计划（SS2012AA020502,2011AA02A114,2014AA020900,2014AA020607）;重大国际合作项目（2014DFB30020）;国家自然科学基金（31470809,31170780）;北京市自然科学基金（5152008）

摘　　要：肝细胞癌以其异质性强、早期诊断难、预后不良和致死率高等特点，成为危害我国国民健康的重要疾病。准确的早期诊断对于提高肝癌切除手术的成功率和降低术后复发与转移至关重要，其关键是早期诊断生物标志物的筛选和验证。以同位素标记相对和绝对定量技术、基于预定同位素标记氨基酸的细胞培养技术等为代表的定量蛋白质组学技术是蛋白质组学技术重要的组成部分，其中前者因其通量高、定量精度高、不受样品来源限制等优势成为定量蛋白质组学技术中的支柱型技术。现就该技术在肝细胞癌发生、发展过程的分子机制及其标志物的筛选研究进展进行综述，为深入理解肝细胞癌发生发展的分子机制和这些新发现的生物标志物的开发创造条件。Due to the features of strong heterogeneity, difficult early diagnosis, poor prognosis, and high fatality rate, hepatocellular carcinoma （HCC） has become an important disease which threatens the health of the Chinese population. Accurate early diagnosis is crucial to improving the success rate of liver cancer resection and reducing postoperative recurrence and metastasis, and its core is the screening and validation of biomarkers for early diagnosis. Isobaric tags for relative and absolute quantitation （iTRAQ） and stable isotope labeling with amino acids in cell culture are important parts of proteomics technology, and iTRAQ has become the most important technique in quantitative proteomics technology due to its advantages of high throughput, high quantitative accuracy, and no limitation by sample source. This article reviews the research advances in molecular mechanism of the development and progression of HCC and screening of markers, in order to establish a theoretical foundation for in-depth understanding of the molecular mechanisms of the development and progression of HCC and the development of new biomarkers.

关 键 词：肝肿瘤 蛋白质组学 同位素标记 肿瘤标志物 同位素标记相对和绝对 定量技术 

分 类 号：R735.7[医药卫生—肿瘤]