胃癌组织中长链非编码RNA STCAT3的表达及其对胃癌细胞恶性表型的作用  

Expression of long noncoding RNA STCAT3 in gastric cancer tissues and its effect on malignant phenotype of gastric cancer cells

作　　者：张健锋[1] 孙中尚 张钱峰 丁伟峰[2] 吴信华[3] 毛振彪[1] 

机构地区：[1]南通大学附属医院消化科,江苏省南通市226001 [2]南通大学附属医院医学检验科,江苏省南通市226001 [3]南通大学附属医院外科综合实验室,江苏省南通市226001

出　　处：《中华医学杂志》2016年第46期3735-3740,共6页National Medical Journal of China

摘　　要：目的探讨长链非编码RNA（lncRNA）胃癌相关转录因子3（STCAT3）在胃癌组织与对应癌旁组织、人胃癌细胞系与正常胃黏膜上皮细胞系中的差异表达，分析其表达水平与胃癌临床病理学特征和肿瘤细胞恶性表型的关系。方法采用实时荧光定量PCR法检测2014年11月至2015年3月南通大学附属医院普外科住院的胃癌患者的胃癌组织与对应癌旁组织中lncRNA STCAT3分子表达水平，分析胃癌组织中lncRNA STCAT3表达水平与临床病理学特征的关系。在lncRNA STCAT3低表达和高表达胃癌细胞系中分别转染表达质粒和干扰质粒转以模拟功能获得及功能缺失实验，以CCK－8及克隆形成实验检测细胞增殖能力，划痕试验检测细胞迁移能力，Transwell小室细胞实验检测细胞侵袭能力，最后通过裸鼠移植瘤实验检测lncRNA STCAT3对成瘤能力的影响。结果胃癌组织中lncRNA STCAT3表达水平显著高于癌旁组织（12.55±0.16比6.52±0.14），癌组织lncRNA STCTA3表达量中间值与癌旁组织相比高6.03倍（P〈0.05）。lncRNA STCAT3在胃癌组织中的表达水平与年龄、性别无相关性（均P〉0.05），但与TNM分期等相关（P〈0.05）。抑制lncRNA STCAT3在胃癌细胞系BGC－823表达后，其克隆形成、增殖、侵袭、迁移能力均明显抑制，差异均有统计学意义（均P〈0.05），上调lncRNA STCAT3在胃癌细胞系AGS表达后，其细胞增殖、克隆形成、迁移、侵袭能力均明显增强，差异均有统计学意义（均P〈0.05）。结论lncRNA STCAT3对胃癌细胞的生长、侵袭有促进作用，表明lncRNA STCAT3的表达失调可能参与了胃癌的发生、发展，提示lncRNA STCAT3可能作为判断胃癌进展的分子标志物及靶向治疗的分子靶点，其分子机制有待进一步研究。Objective To detect the expression of long noncoding RNA（lncRNA）stomach cancer-associated transcript-3（STCAT3） in gastric cancer tissues, adjacent tissues, human gastric cancer cell lines and normal gastric epithelial cell lines, and to investigate the relationship between STCAT3 expression and clinicopathological features and malignant phenotype of gastric cancer.Methods Quantitative fluorescent real-time polymerase chain reaction （qRT-PCR） was applied to detect the lncRNA STCAT3 expression levels in gastric cancer tissues, paired adjacent non-tumorous tissues, in order to explore the relationship between STCAT3 expression and clinicopathological features of gastric cancer. lncRNA STCAT3 low-expressing and high-expressing gastric cancer cell lines were transfected with expression plasmid to simulate gain-of-function, or interference plasmid to achieve loss-of-function. Cell proliferation was measured with CCK-8 and colony formation assay, cell migration with scratch assay, and cell invasion with Transwell migration assay. human gastric tumor were also transplanted to nude mice to detect the effect of lncRNA STCAT3 on tumorigenesis.Results The expression of lncRNA STCAT3 was generally up-regulated in gastric cancer tissues compared with the adjacent tissues（12.55±0.16 vs 6.52±0.14）, with median expression level in gastric cancer tissues being 6.03 higher （P〈0.05）.Meanwhile, the expression level of lncRNA STCAT3 in gastric cancer tissues was not correlated with age or gender （both P〉0.05）, while positively correlated with TNM stage （P〈0.05）. Interference of lncRNA STCAT3 expression in BGC-823 cells was found associated with significantly suppressed colony formation, proliferation, invasion, and migration （all P〈0.05）. Over-expression of lncRNA STCAT3 in AGS cells were also founded could promote the gastric cancer cells′ proliferation, colony formation, migration, and invasion （all P〈0.05）.Conclusions lncRNA STCAT3 may participate in the proliferation and invasion o

关 键 词：胃肿瘤 RNA 长链非编码RNA 胃癌相关转录因子3 

分 类 号：R735.2[医药卫生—肿瘤]