乌塌菜与薹菜变种间杂交后代硫代葡萄糖苷组分及含量分析  

Analysis of Glucosinolates Composition and Content in Filial Lines Derived from Inter-Varietal Cross of Savoy and Tai-tsai by HPLC

作　　者：冯希环[1] 孙艳[1] 刘倩倩[1] 汪俏梅[2] 刘维信[1] 

机构地区：[1]青岛农业大学园艺学院青岛市园艺植物遗传改良与育种重点实验室,山东青岛266109 [2]浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室,浙江杭州310029

出　　处：《中国蔬菜》2016年第12期42-46,共5页China Vegetables

基　　金：国家自然科学基金项目(31401864);山东省自然科学基金项目(ZR2016CM15);青岛市自主创新计划应用基础研究项目(16-5-1-74-jch)

摘　　要：以乌塌菜品种上海小八叶、薹菜品种平度薹菜及变种间杂交后代6-1和6-5为试材,采用高效液相色谱法(HPLC)分析叶片及叶柄中硫代葡萄糖苷的组分与含量,以期了解不结球白菜变种间杂交后代的硫苷变化。结果表明:4份材料的叶片、叶柄中均检测到组分相同的脂肪族硫苷、吲哚族硫苷各4种,而芳香族硫苷只在变种间杂交后代6-1的叶片和叶柄中检测到;参试各材料叶片中总脂肪族硫苷含量、总吲哚族硫苷含量和总硫苷含量均高于叶柄。变种间杂交后代6-1叶片、叶柄中有益人体健康的2-苯乙基硫苷(NAS)和吲哚-3-甲基硫苷(GBC)含量均高于亲本上海小八叶和平度薹菜,表明变种间杂交可以用于改良不结球白菜硫苷组分及含量。To understand the variation of glucosinolates composition in backcrossing lines derived from inter-varietal crosses in pakchoi, the content and composition of glucosinolates were analyzed by high performance liquid chromatography （ HPLC ） for savoy [ Brassica campestris L. ssp. chinensis （ L. ） Makino var. rosularis Tsen et Lee ]cv. ‘Shanghaixiaobaye＇, tai-tsai [ Brassica campestris L. ssp. chinensis （ L. ） Makino var. tai-tsai Hort ]cv. ‘Pingdutaicai＇, and 2 lines selected from F2 populations of 4 times backcrossing of （ ‘Shanghaixiaobaye＇ × ‘Pingdutaicai’ ） with ＇Shanghaixiaobaye＇ as the recurrent parent. The results showed that each of 4 kinds of aliphatic and indole glucosinolate were detected in the leaf and petiole of 4 tested materials. The total contents of aliphatic glucosinolate, indole glucosinolate and total glucosinolate in leaves were higher than petioles, and also different with 4 tested materials. The contents of health-beneficial compounds 2-phenylethyl glucosinolate （ NAS ） and glucobrassicin （ GBC ） were higher in leaves of ‘6-1＇ than both its parents ‘Shanghaixiaobaye＇ and ‘Pingdutaicai＇, indicating that inter-varietal crosses could be used in breeding new pakchoi cuhivars with improved glucosinolate composition.

关 键 词：乌塌菜 薹菜 变种间杂交 硫代葡萄糖苷 高效液相色谱法 

分 类 号：S634[农业科学—蔬菜学]