X线照射剂量率对A549肺癌细胞周期的影响  

The effects of dose rate of X-ray on cell cycle distribution of A549 lung cancer cells

作　　者：宋淑军 左晓勇 徐冰心 王莹 单改仙 任晔 司少艳 王宗烨 

机构地区：[1]解放军第三〇六医院特种医学实验研究中心,北京1001010 [2]解放军第三〇六医院放疗中心,北京1001010

出　　处：《癌症进展》2016年第10期970-972,976,共4页Oncology Progress

摘　　要：目的了解不同剂量率X线照射对非小细胞肺癌细胞周期的影响,以期为临床制定放疗计划提供一定的实验依据。方法选取A549肺癌细胞进行培养48 h,采用直线加速器行X线照射,照射剂量为6 Gy,照射剂量率分别为1、2、4、6 Gy/min,照射后24 h收集细胞,用70%乙醇固定,RNA酶处理,碘化丙啶染色,采用流式细胞学的方法检测细胞周期。结果 X线照射后细胞周期分布发生明显的改变,细胞在G2/M期发生明显阻滞,S期的细胞明显减少,其中以2 Gy/min照射的细胞G2/M期的阻滞最为明显。结论 X线照射影响A549细胞周期的进程,相同的剂量,不同的剂量率对细胞周期的影响存在明显差异。选取最佳剂量率进行治疗,可能有助于提高肿瘤放射治疗效果。Objective To investigate the effects of dose rate of X-ray on cell cycle of non-small-cell lung cancer （NSCLC） cells. Method A549 cells of a NSCLC cell line were cultured for 48 hours and then irradiated with X-rays at dose of 6 Gy from a linear accelerator. The dose rates were 1, 2, 4 and 6 Gy/min, respectively. Cells were collected 24 hours after irradiation, and then were fixed with 70%ethanol, treated with RNase and stained with propidium iodide. Cell cycle distribution was analyzed using BD FACSCalibur. Result Cell cycle distribution in irradiated cells was altered sig-nificantly. Radiation induced cell cycle arrest in G2/M phase and cells decreased in S phase. The G2/M phase arrest was most significant in cells irradiated at dose rate of 2 Gy/min. Conclusion X-ray irradiation influences the cell cycle distri-bution of A549 cells. Though at same radiation dose, different dose rates impose varied effects on cell cycles. Thus, treat-ment with optimal dose rate would improve the efficacy of radiotherapy.

关 键 词：X线 剂量率 细胞周期 放射治疗 

分 类 号：R734.2[医药卫生—肿瘤]