Expression,Purification,Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase(NDK)from Aspergillus Flavus  

作　　者：王森 李博文 江龙光 汪世华 张彦丰 王宇 

机构地区：[1]Key Laboratory of Biopesticide and Chemical Biology of Education Ministry,Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province,School of Life Sciences,Fujian Agriculture and Forestry University [2]State Key Laboratory of Structural Chemistry,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences

出　　处：《Chinese Journal of Structural Chemistry》2016年第11期1708-1713,共6页结构化学（英文）

基　　金：supported by National 973 Program(No.2013CB127802)of Ministry of Science and Technology of China;the National Natural Science Foundation of China(Nos.31172297 and 31400100)

摘　　要：Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human, Structural and functional studies ofA. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to 〉98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 A^° resolution. The crystal of NDK is in space group of C121 with a = 190.84, b = 169.47, c = 146.94 A^°. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human, Structural and functional studies ofA. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to 〉98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 A^° resolution. The crystal of NDK is in space group of C121 with a = 190.84, b = 169.47, c = 146.94 A^°. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.

关 键 词：AspergUlusflavus nucleoside diphosphate kinase EXPRESSION PURIFICATION CRYSTALLIZATION 

分 类 号：R99[医药卫生—毒理学]