亚硒酸钠对胡萝卜愈伤组织诱导形成的影响  

Effect of Sodium Selenite on the Induction of Carrot Callus

作　　者：武晓英[1] 王青[1] 乔宏萍[1] 郝雪峰[1] 吴丽华[1] 郝彩霞[1] 曹贵东 

机构地区：[1]太原师范学院生物系,山西太原030031 [2]山西新福瑞沃生物技术工程有限责任公司,山西太原030012

出　　处：《太原师范学院学报（自然科学版）》2016年第3期91-96,共6页Journal of Taiyuan Normal University:Natural Science Edition

基　　金：国家自然基金(21307087);山西省自然基金(2014011028-4)

摘　　要：选取新鲜胡萝卜的形成层为试验材料,研究亚硒酸钠对胡萝卜愈伤组织诱导形成的影响.在添加2.0mg/L 2,4-D和0.8mg/L 6-BA的MS培养基中分别加入0,0.5,1.0,2.0mg/L的亚硒酸钠诱导培养愈伤组织,观察愈伤组织的诱导情况并计算愈伤组织的诱导率,同时测定抗氧化能力的相关指标,包括总抗氧化能力、GSH-PX活力、SOD活力、H_2O_2浓度、MDA含量.实验结果表明:亚硒酸钠浓度为0.5mg/L时,胡萝卜愈伤组织被有效诱导,愈伤组织生长状况最佳,抗氧化能力显著提高.Fresh Carrot cambium selected as experimental materials to study the effect of sodium selenite on the induction of Carrot callus. Added the concentration of sodium selenite 0, 0. 5, 1.0, 2.0 mg/L, respectively, into MS medium with 2.0 mg/L 2, 4-D and 0.8 mg/L 6-BA to observe the induction of callus, to calculate the induction rate of callus and to determinate the antioxidant capacity, namely, total antioxidant capacity, GSH-PX and SOD activity, H2O2 and MDA concentration. The results show that the optimal concentration of sodium selenite added in MS medium is 0. 5 mg/L. Carrot callus are induced effectively, growth condition of callus are improved, the antioxidant capacity of callus are significantly increased.

关 键 词：亚硒酸钠 愈伤组织 抗氧化能力 

分 类 号：Q657[生物学—生物物理学]