缺氧通过下调miR-132表达促进前列腺癌细胞的体外生长和侵袭  

Hypoxia promotes the growth and invasiveness of prostate cancer cells by down-regulating miR-132 in vitro

作　　者：查娟民[1] 徐晓峰[2] 李大鹏[1] 桂琦[1] 梁容瑞[1] 周秀敏[1] 

机构地区：[1]苏州大学附属第一医院肿瘤内科,江苏苏州215006 [2]南京军区南京总医院泌尿外科,江苏南京210002

出　　处：《中华男科学杂志》2016年第12期1071-1076,共6页National Journal of Andrology

基　　金：国家自然科学基金(81301906;30901716)~~

摘　　要：目的：探讨miR-132在前列腺癌中的表达及其对前列腺癌细胞生长和侵袭的调节作用,以及缺氧条件下前列腺癌细胞中miR-132水平的变化及其生物学行为的改变。方法：荧光定量PCR分析前列腺癌组织中miR-132的表达水平,分析其与临床分期和Gleason评分的关系,及体外缺氧对人前列腺癌细胞株PC3中miR-132表达的影响;磺酰罗丹明B（SRB）比色法和Matrigel侵袭实验分别检测缺氧和miR-132 mimic质粒转染对PC3细胞活力和侵袭的体外影响。结果：相对于对照组癌旁组织,前列腺癌组织中miR-132水平降低至对照组的52.38%（T1-T2期）（P〈0.01）和21.59%（T3-T4期）（P〈0.01）。缺氧培养下,PC3细胞的miR-132水平明显降低（P〈0.01）。miR-132 mimic质粒转染48 h和72 h后,PC3细胞活力显著降低（P〈0.05或P〈0.01）;转染后48 h,PC3细胞侵袭力降低了57.5%（P〈0.01）。然而,miR-132 mimic质粒转染PC3细胞后,常氧和缺氧培养两种方式间的细胞活力和侵袭力均无明显差异（P〉0.05）。结论：miR-132的表达降低与前列腺癌的临床分期和Gleason评分密切相关;缺氧通过下调miR-132表达,可在体外促进前列腺癌细胞的活力和侵袭,可能进一步促进前列腺癌的生长和转移。Objective： To explore the expression of miR-132 in prostate cancer and its effects on the growth and invasiveness of prostate cancer cells and the influence of hypoxia on the level of miR-132 and biological behavior of prostate cancer cells. Methods ： Real time PCR was used to measure the expression level of miR-132 in the prostate cancer tissue, analyze its relationship with the clinical stage and Gleason score of prostate cancer, and determine the influence of hypoxia on the miR-132 level in the human prostate cancer PC3 cell line in vitro. Sulfor-hodamine B chromatometry and Matrigel invasion assay were employed to detect the effects of hy-poxia and miR-132 mimic plasmid transfection on the viability and invasiveness of PC3 cells in vitro. Results： The miR-132 level in the prostate cancer was significantly declined to 52.38% （in T1-T2 stages） and 21.59% （in T3 -T4 stages） of that in the cancer adjacent tissue （both P 〈 0.01 ）. In hypoxia, the expression of miR-132 was significantly decreased in the PC3 cells （P 〈 0.01 ）. After 48 and 72 hours of transfection with miR-132 mimic plasmid, the viability of the PC3 ceils was markedly reduced （ P 〈0.05 or P 〈 0.01 ）, and their invasiveness decreased by 57.5% after 48 hours （P 〈 0.01 ）. However, there was no significant difference in the viability or invasiveness of the PC3 cells transfected with miR-132 mimic plasmid between normoxia and hypoxia. Conclusion ： The reduced expression of miR-132 is closely related to the clinical stage and Gleason score of prostate cancer. Hypoxia increases the viability and invasiveness of prostate cancer cells in vitro by down-regulating the expression of miR-132 and consequently may promote the growth and metastasis of prostate cancer.

关 键 词：miR-132 缺氧 前列腺癌 生长 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]