高危型人乳头瘤病毒感染的子宫颈鳞状上皮组织中p16INK4α蛋白表达及其意义  

Expression of p16INK4α in cervical squamous epithelium infected by high-risk HPV and its significance

作　　者：王丽凤 韩蓉晖[2] 李丽 刘玲玲 吴翠玲 胡丽娜 张振华 

机构地区：[1]山西大医院病理科,太原030032 [2]山西医科大学第一医院妇科二病区,太原030001

出　　处：《肿瘤研究与临床》2016年第11期757-760,共4页Cancer Research and Clinic

基　　金：山西省科技产业化环境建设项目（2013072024）

摘　　要：目的：研究高危型人乳头瘤病毒（HPV）感染的子宫颈鳞状上皮组织中p16INK4α蛋白表达情况，探讨其在子宫颈鳞状上皮癌变过程中的作用。方法应用HC2法检测子宫颈高危型HPV-DNA，收集HPV阳性的慢性子宫颈炎组织31例、子宫颈上皮内瘤变（CIN）组织48例（CINⅠ级16例、CINⅡ级14例、CINⅢ级18例）及鳞状细胞癌组织48例，采用免疫组织化学法检测各组p16INK4α蛋白表达情况。结果慢性子宫颈炎组、CINⅠ级组、CINⅡ级组、CINⅢ级组及鳞状细胞癌组中p16INK4α蛋白阳性率分别为0（0／31）、25%（4／16）、100%（14／14）、100%（18／18）及100%（48／48）。 CINⅡ级及以上病变组中p16INK4α蛋白阳性率明显高于慢性子宫颈炎组及CINⅠ级组（P＜0.05）。结论 p16INK4α蛋白阳性表达可反映鳞状上皮异型增生，对高危型HPV感染的子宫颈鳞状上皮内病变的准确诊断具有重要参考价值。Objective To investigate p16INK4α protein expression in cervical squamous epithelium infected by high-risk HPV, and to explore the role and significance of p16INK4α in carcinogenesis. Methods High-risk HPV DNA were detected by HC2 methods. 31 cases of chronic cervicitis, 48 cases of cervical intraepithelial neoplasia （CIN） （including 16 cases of CINⅠ, 14 cases of CINⅡ, 18 cases of CINⅢ） and 48 cases squamous cell carcinoma （SCC） cervical tissues with high-risk HPV-positive were collected. p16INK4αprotein was detected by immunohistochemistry. Results The positive rates of p16INK4α were 0 （0/31）, 25 %（4/16）, 100 % （14/14）, 100 % （18/18） and 100 % （48/48） in chronic cervicitis, CINⅠ, CINⅡ, CINⅢ and SCC group, respectively. The positive rates of CINⅡ , CINⅢ and SCC groups were significantly higher than those of chronic cervicitis and CINⅠ groups （P〈0.05）. Conclusion The positive expression of p16INK4αprotein indicates cervical squamous epithelium dysplasia, which is the important reference in accurate diagnosis of cervical squamous intraepithelial lesions infected by high-risk HPV.

关 键 词：人乳头瘤病毒 子宫颈上皮内瘤样病变 子宫颈肿瘤 蛋白p16INK4α 

分 类 号：R737.33[医药卫生—肿瘤]