基于表面增强拉曼光谱的肝病血清检测技术研究  

Serum detection technology of liver diseases based on surface-enhanced Raman spectroscopy

作　　者：邵丽婷[1] 肖瑞[1] 王升启[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2016年第11期888-891,共4页Military Medical Sciences

基　　金：国家自然科学基金重点资助项目(81230089);北京市科学技术委员会资助项目(Z161100000116040)

摘　　要：目的通过比较正常人与慢性乙肝、肝硬变和肝癌患者血清之间的拉曼光谱差异,探索基于表面增强拉曼散射(SERS)的血清诊断新方法。方法首先制备纳米银溶胶作为活性基底,分别测量30例正常人和48例慢性乙肝、48例肝硬变、46例肝癌患者血清的增强拉曼信号,并进行正交偏最小二乘法判别分析(OPLS-DA)。结果正常人与3组肝病患者血清在位移625、725、806、947、1018、1219、1131、1329、1440、1580、1660 cm-1处均有拉曼峰,且峰强弱存在差异;正常人血清在位移1096和1395 cm-1处有强峰,肝病患者血清在位移887 cm-1有强峰。ROC曲线下面积(AUC)分别为0.981、0.966、0.984。结论初步研究表明血清SERS图谱可作为肝病早期诊断的一种辅助手段。Objective To explore a new method of serodiagnosis based on surface-enhanced Raman spectroscopy （SERS）by using SERS of blood serum to discriminate chronic hepatitis B ,liver cirrhosis and liver cancer patients from normal people .Methods Ag nanoparticles were prepared as the active substrate , and SERS signals of serum taken from 30 healthy people ,48 patients of chronic hepatitis B ,48 patients of liver cirrhosis and 46 liver cancer patients were measured before the data were finally exported into SIMCA for orthogonal partial least squares discriminant analysis （ OPLS-DA ） . Results Peaks at wavenumbers 625, 725, 806, 947, 1018, 1219, 1131, 1329, 1440, 1580 and 1660 cm-1 could be consistently observed in all the four groups ,and the intensity of peaks differed in each group .In comparison with patients , the intensities at 1096 and 1395 cm-1 were stronger for the controls .A strong enhancement in the intensity of the peak at around 887 cm-1 was observed in the spectra of thepatients′serum.The integration area under curve values （ AUC） of ROC was 0.981,0.966 and 0.984 for chronic hepatitis B, liver cirrhosis and liver cancer patients ,respectively.Conclusion This preliminary study demonstrates that serum SERS can be used as a complementary method in the early diagnosis of liver cancer.

关 键 词：光谱分析 拉曼 血清学试验 肝炎 慢性 肝硬化 肝肿瘤 正交偏最小二乘法判别分析 早期诊断 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学] R575.2