乙醇胺接枝聚天冬氨酸仿生低温保护剂的合成及性能  

Synthesis and cryoprotective performances of poly(aspartic acid)grafted from ethanol amine

作　　者：单飞[1] 王亚兵[1] 刘婷[1] 冯涛[1] 彭刚[1] 余定华[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《南京工业大学学报（自然科学版）》2016年第6期32-38,共7页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家科技支撑计划(2012BAD32B08);国家自然科学基金(21376120)

摘　　要：基于抗冻蛋白的结构和功能特点,合成了仿生低温保护剂乙醇胺接枝聚天冬氨酸。利用红外光谱(FTIR)和核磁共振氢谱(1H NMR)分析聚合物结构,通过非等温差示扫描量热法(DSC)分析聚合物的抗冻性能并利用光学显微镜观察在低温下聚合物溶液冰晶形态。实验结果表明,聚合物具有良好的抗冻性能。接枝率较高时,具有较好的热滞活性,冰点降低程度较大,冰晶体积较小且较圆润。接枝率80%时,热滞活性最高,达0.44℃,保护剂质量浓度存在阈值为5 mg/mL。该仿生低温保护剂为现代低温保护技术提供了一种新的可能。Based on the structure and function of antifreeze protein, poly （ aspartic acid ） grafted from ethanol amine was synthesized and used as biomimetic cryoprotective agent. The structure was characterized by Fourier transform infrared spectroscopy （ FT-IR ） and nuclear magnetic resonance hydrogen spectrum 1H NMR. The antifreeze performance and morphology of ice crystal were determined by differential scanning calorimetry （ DSC） and optical microscopy. Results showed that these polymers interfered with the crystallization process of water. The differential scanning calorimetry results showed that polymers with higher grafting ratio had better thermal hysteresis activity and decreased the freezing point. Meanwhile,the ice crystal showed the smaller and rounded particle morphology. It was exhibited that the grafting rate at 80% had the highest thermal hysteresis activity （ 0.44℃） and the cryoprotective agent concentration threshold was 5 mg/mL. Results demonstrated that the synthetic biomimetic polymer could be used as a cryoprotective agent.

关 键 词：聚天冬氨酸 乙醇胺 低温保护剂 热滞活性 冰晶形态 

分 类 号：TQ317[化学工程—高聚物工业]