几种典型扫描电镜生物样本制备  

Several Biological Typical Samples Preparation Methods of Scanning Electron Microscope

作　　者：胡春辉[1] 徐青[2] 孙璇[2] 于浩[2] 袁玉清[1] 

机构地区：[1]青岛农业大学中心实验室,山东青岛266109 [2]青岛农业大学生命科学学院,山东青岛266109

出　　处：《湖北农业科学》2016年第20期5389-5392,5402,共5页Hubei Agricultural Sciences

基　　金：青岛农业大学实验技术课题资助项目(SYJK12-03);青岛农业大学大学生创新项目

摘　　要：总结了几种典型扫描电镜生物样本的制备方法。结果表明,分别采用牙签直接涂抹、在合适的溶剂中超声分散后用铜片和铜网捞取的方法,得到较好的粉末样品扫描电镜图像;微生物在液体或固体培养基中培养后,经戊二醛固定,乙醇脱水和冷冻干燥等处理过程;植物样品根据含水量的不同,选择合适的固定液和干燥方法对样品进行处理;动物样品采用双固定和临界点干燥的方法进行处理;均获得较为理想的扫描电镜图片。This paper explores and summarizes the Scanning Electron Microscope（SEM） preparation methods of several typical samples. The results showed that using toothpick direct smear method,copper and copper net gain by ultrasonic dispersion in suitable solvent to obtain excellent SEM images;Microorganisms in liquid or solid culture were fixed in glutaraldehyde using ethanol dehydration and freeze drying process to prepare the samples;Plant samples under different water content,select the applicable stationary liquid and proper drying methods for the processing of samples;Animal samples were prepared by using the method of double fixed and critical point drying processing; Above all samples are obtain ideal scanning electron microscope images.

关 键 词：生物样品 扫描电镜 样品制备 干燥方法 场发射 

分 类 号：TB383[一般工业技术—材料科学与工程]