麒麟尾总黄酮提取及其抗氧化作用研究  

Extraction of Total Flavone in Epipremnum pinnatum and Its Antioxidation

作　　者：罗泽萍[1] 潘立卫[1] 

机构地区：[1]河池学院化学与生物工程学院/广西高校微生物及植物资源开发利用重点实验室,广西宜州546300

出　　处：《湖北农业科学》2016年第18期4783-4786,共4页Hubei Agricultural Sciences

基　　金：河池学院校级青年科研课题项目(2014QN-N002);生物化工广西重点学科(河池学院)建设基金项目(201308)

摘　　要：利用单因素试验及正交试验法考察麒麟尾(Epipremnum pinnatum)总黄酮提取的最佳工艺,并通过测定总黄酮对羟基自由基(·OH)和2,2-二(4-叔辛基)-1-苦肼基自由基(DPPH·)的清除作用研究其抗氧化性。结果表明,麒麟尾总黄酮提取的最佳工艺条件为超声时间35 min、超声温度90℃、料液比1∶15(g∶m L)、乙醇体积分数85%,在此条件下总黄酮得率为3.04%;抗氧化试验结果表明,麒麟尾总黄酮具有较强的抗氧化活性,对·OH和DPPH·自由基的IC50分别为2.355和0.143 mg/m L。The optimum extraction conditions of total flavone were established through single-factor test and orthogoganal test;besides,the antioxidant activity was assessed by measuring ·OH and DPPH· radicals scavenging capacity.Result showed that the optimal extraction conditions of total flavone in Epipremnum pinnatum were as following：The ultrasonic time was 35 min,the ultrasonic temperature was 90 ℃,the material / water ratio was 1∶15 g / m L,and the ethanol / water was 85%,the yield of total flavone in the optimized conditions was 3.04%; The antioxidant activity test showed that total flavone of Epipremnum pinnatum had strong antioxidant activity with the half scavenging rate（IC50）of ·OH and DPPH· radicals were 2.355,0.143 mg / m L respectively.

关 键 词：麒麟尾(Epipremnum pinnatum) 总黄酮 提取工艺 抗氧化活性 

分 类 号：S567.79[农业科学—中草药栽培] R284.2[农业科学—作物学]