CUEDC2通过活化PI3K/Akt信号通路促进乳腺癌细胞的生长  

CUEDC2 promotes breast cancer progression through activation of PI3K/ Akt signaling pathway

作　　者：李彦姝[1] 高云玲[1] 张红艳[1] 汤丽娜[1] 刘芙蓉[1] 李丰[1] 

机构地区：[1]中国医科大学基础医学院细胞生物学教研室,教育部医学细胞生物学重点实验室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2017年第2期183-185,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:31371424)

摘　　要：目的:探讨CUE结构域2(CUE domain-containing 2,CUEDC2)蛋白在人乳腺癌细胞中的生物学作用。方法:人乳腺肿瘤细胞MCF-7转染的Myc-CUEDC2真核表达载体,提取转染细胞的总蛋白检测磷酸化Akt和总Akt的表达变化。利用CCK-8检测过表达CUEDC2后MCF-7细胞的生长。结果:CUEDC2能够活化Akt,使磷酸化Akt表达增加,能够促进人乳腺癌细胞生长。结论:当乳腺癌中CUEDC2表达增高时,能够引起PI3K/Akt信号通路活化,促进肿瘤细胞生长。Objective：To evaluate the biological role of CUEDC2 in human breast cancer cells.Methods：Human breast cancer cell line MCF-7 were transient transfected with Myc-CUEDC2 constructs,and whole-cell lysates were extracted with the level of phosphorylated Akt（ p-Akt） and the total Akt proteins.CCK-8 assay was used for detecting the role of CUEDC2 in breast cancer cell line progression.Results：CUEDC2 induced an increase in the level of phosphorylated Akt（ p-Akt） and increased the growth of breast cancer cells.Conclusion：Our results suggest that increased expression of CUEDC2 contributes to tumor growth by activation of the PI3 K / Akt pathway in breast cancer cell lines,which may be one of the mechanisms that attribute to promote the growth of cancer cells.

关 键 词：乳腺肿瘤 CUEDC2 细胞生长 

分 类 号：R737.9[医药卫生—肿瘤]