沉默ATM基因对人胃癌细胞BGC823放射敏感性的影响  

Effect of silencing of ATM expression by siRNA on radiosensitivity of human gastric adenocarcinoma BGC823 cells

作　　者：郑旭[1] 刘丹[1] 刘帆[1] 

机构地区：[1]中国医科大学附属第四医院放疗科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2017年第2期190-194,共5页Journal of Modern Oncology

摘　　要：目的:探讨毛细血管扩张突变基因(ATM)对人胃癌细胞放射敏感性的影响。方法:将ATM基因敲减后获取得到的稳定转染克隆细胞和空载细胞同时通过不同剂量的照射后,观察敲减ATM基因前后BGC823细胞平板克隆形成率,并使用流式细胞术,观察ATM被敲减后对细胞周期和凋亡的影响。结果:BGC823细胞中的ATM被敲减后,经不同剂量的照射(2、4、6和8Gy)克隆形成率分别为(15.4±0.9)%、(9.2±0.3)%、(2.2±0.1)%和(1.0±0.1)%,远低于空载细胞的克隆形成率分别为(28.1±0.6)%、(15.6±0.8)%、(5.3±0.1)%和(1.8±0.1)%,P均小于0.05,有显著性差异。采用相同剂量(4Gy)照射后,流式细胞术检测发现,BGC823细胞中的ATM被敲减后,细胞周期被阻滞,细胞停止生长,并在12h后出现凋亡的现象。结论:ATM基因的表达可影响胃癌细胞的放射敏感性,ATM的表达被敲减后对BGC823细胞的放射起到增敏作用。ATM基因在胃癌放射敏感性方面可能有非常重要的作用。Objective：To study the effect of interference of ataxia-telangiectasia mutated（ ATM） expression of the radiosensitivity on human gastric cancer BGC823 cells.Methods：Using the plasmid of interfering RNA to transfect BGC823 cells.The mRNA and protein expression level of ATM was measured by RT-PCR,Real-time PCR and Western blot,respectively.Using the colonies formation assay to observe the change of cell radiosensitivity by different doses of irradiation.Cell cycle and cell apoptosis were analyzed by flow cytometry.Results：The expression of ATM in the BGC823 cells were knocked out by small interfering RNA（ siRNA）,the colonies formation numbers / ratios of BGC823-Ri-ATM cells were（ 15.4 ± 0.9） %,（ 9.2 ± 0.3） %,（ 2.2 ± 0.1） % and（ 1.0 ± 0.1） % by the different doses of irradiation（ 2,4,6,and 8Gy）,which were lower than the BGC823-Vector＇s colonies formation numbers /ratios（ 28.1 ± 0.6） %,（ 15.6 ± 0.8） %,（ 5.3 ± 0.1） % and（ 1.8 ± 0.1） % respectively（ P〈0.05）.The flow cytometry detection that the BGC823-Ri-ATM cells were induced cell cycle arrest and apoptosis by 4Gy intensity radioactivity after 12 h,but not incurred in the vector cells.Conclusion：ATM gene expression can affect the radiation sensitivity of gastric cancer cells,the silencing of ATM increase the radiotherapy sensitization of BGC823 cells.ATM gene may play an important role in gastric cancer radiation sensitivity.

关 键 词：胃癌 毛细血管扩张突变基因 小干扰RNA 放射敏感性 

分 类 号：R735.2[医药卫生—肿瘤]