β-arrestin2基因转染对人肺腺癌细胞A549的凋亡影响  

Apoptosis of human lung adenocarcinoma cells A549 transfected by β-arrestin2 gene

作　　者：胡美琴[1] 龚军[1] 唐昃 高琨[2] 

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)腹盆肿瘤内科,湖北黄石435000 [2]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)脊柱外科,湖北黄石435000

出　　处：《现代肿瘤医学》2017年第2期195-197,共3页Journal of Modern Oncology

基　　金：湖北省黄石市医药卫生科研立项项目(编号:201402)

摘　　要：目的:观察外源性β-arrestin2基因对人肺腺癌细胞株A549凋亡的影响。方法:将真核表达重组体pc DNA3.1-β-arrestin2质粒和空载体pc DNA3.1质粒采用脂质体转染技术分别导入人肺腺癌A549细胞,然后采用G418筛选方法获得稳定转染的细胞系,采用qRT-PCR和Western blot分别在基因水平和蛋白水平检测β-arrestin2基因的表达。采用流式细胞仪分析转染细胞的凋亡情况。结果:经脂质体转染和筛选,建立了稳定表达β-arrestin2基因的A549细胞系。转染β-arrestin2基因的细胞生长速度较未转染组和转染空白载体组相比明显减慢(P<0.05)。结论:β-arrestin2基因可促进人肺腺癌细胞A549的细胞凋亡。Objective：To determine the effects of exogenous β-arrestin2 on human lung adenocarcinoma cells A549.Methods：pc DNA3.1-β-arrestin2 and pc DNA3.1 were introduced into A549 cells by lipofectin transfection,and the A549 cells stably expressing β-arrestin2 gene were established by G418 selection.The expression of β-arrestin2 was detected by qRT-PCR and Western blot.Apoptosis of cells was detected by flow cytometry.Results：A549 cells stably expressing β-arrestin2 protein were established by lipofection mediated transfection and selected for further study.Compared with the nontransfected and vector transfected cells,the positive clone cells grew more slowly（ P〈0.05）.Conclusion：β-arrestin2 gene might promote the apoptosis of A549 cells.

关 键 词：β-arrestin2 肺腺癌 A549细胞 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]