雷公藤内酯醇对淋巴瘤Raji细胞ID4基因及DNA甲基转移酶表达的影响  

Expression of ID4 gene and DNA methyltransferases of triptolide on lymphoma Raji cells

作　　者：蔡静怡 梁亮 王巍 杨志刚 

机构地区：[1]广东医科大学医学检验学院临床血液检验学教研室,广东东莞523808 [2]广东医科大学附属医院血液内科,广东湛江524001 [3]广东医科大学附属医院血液疾病研究室,广东湛江52400

出　　处：《现代肿瘤医学》2017年第2期198-201,共4页Journal of Modern Oncology

基　　金：湛江市科技计划项目(编号:2012C3101031)

摘　　要：目的:探讨雷公藤内酯醇(triptolide,TPL)对淋巴瘤Raji细胞ID4基因及DNA甲基转移酶(DNA methyltransferases,DNMT)mRNA表达的影响。方法:收集经0、20ng/ml TPL处理的Raji细胞,采用荧光定量RT-PCR法检测Raji细胞ID4基因mRNA的表达。经0、5、10、15、20ng/ml TPL处理的Raji细胞,采用荧光定量RT-PCR法检测Raji细胞DNMT1、DNMT3A和DNMT3BmRNA的表达。结果:未经TPL处理的Raji细胞ID4基因无表达,20ng/ml TPL作用后ID4基因表达增强。在0~20ng/ml浓度范围内,随着TPL浓度的增加,DNMT1、DNMT3A和DNMT3B表达下降并呈浓度依赖性。结论:TPL可抑制Raji细胞DNA甲基转移酶mRNA,恢复Raji细胞ID4基因mRNA表达。Objective：To study the effect of triptolide（ TPL） on expression of ID4 and DNA methyltransferases（ DNMT） mRNA on lymphoma Raji cells.Methods：Afer treated with 0,20ng/ml TPL for 24 h,the Raji cells＇ mRNA expression of ID4,DNMT1,DNMT3 A,DNMT3Bwas detectected by Fluorescent quantitative RT-PCR.Afer treated with 0,5,10,15,20 ng / ml TPL for 24 h,the Raji cells＇ mRNA expression of DNMT1,DNMT3 A,DNMT3Bwas detectected by Fluorescent quantitative RT-PCR.Results：ID4 gene failed to express in Raji cells without TPL.The expression was recovered after the cells exposed to 20 ng / ml TPL.TPL could significantly down-regulate DNMT1,DNMT3 A and DNMT3 Bgene on mRNA level in a dose dependent manner.Conclusion：TPL can inhibit the Raji cell DNA methyl transferase,activate the expression of ID4 mRNA.

关 键 词：ID4 雷公藤内酯醇 RAJI细胞 DNA甲基转移酶 

分 类 号：R733.4[医药卫生—肿瘤]