双歧杆菌的分子生物学鉴定方法的比较  

The molecular identification of bifidobacterium

作　　者：熊强[1] 王延斌[1] 韩浩[1] 

机构地区：[1]南京工业大学食品与轻工学院,江苏南京210009

出　　处：《食品与发酵工业》2016年第12期45-48,共4页Food and Fermentation Industries

摘　　要：利用实验室设计的双歧杆菌特异性引物,采用降落聚合酶链式反应(Polymerase Chain Reaction,PCR)的方法对供试菌株的16S r DNA进行扩增,比较细菌分别通用引物和双歧杆菌特异性引物对双歧杆菌属不同菌株及参考菌株扩增效果的影响。实验结果表明,细菌通用引物对不同双歧杆菌进行扩增,扩增出来的条带不清晰,而用双歧杆菌特异性引物进行扩增,扩增出来的条带清晰。因此,该方法可以准确地鉴定双歧杆菌,为双岐杆菌的鉴定提供了理论依据。In this paper, the specific primers for bfidobacterium designed by our laboratory was combined the touch-down PCR to amplify 16S rDNA of test strains. The amplification effects of the universal primers for bacteria and the specific primers for bifidobacterium on different strains of the genus bifidobacterium and reference strains were compared. The experimental results showed that the amplification of sample from different bifidobacteria using univer- sal primers for bacteria resulted in unclear bands, while amplification with specific primers for bifidobacterium gave rise to very bright bands. Therefore, this method can accurately identify the bifidobacterium and our work provides a theoretical basis for the identification of bifidobacterium.

关 键 词：双歧杆菌 聚合酶链式反应 分子生物学 鉴定 

分 类 号：Q93-331[生物学—微生物学]