DUSP10对人卵巢癌A2780细胞迁移及侵袭的影响  

The effect of DUSP10 on migration and invasion of ovarian cancer A2780 cells

作　　者：彭诗元 葛元勋 姚丽[2] 张佳瑞[2] 王淑妹[2] 兰淼[2] 张伟[2] 李艳红 

机构地区：[1]第四军医大学唐都医院妇产科,陕西西安710038 [2]第四军医大学唐都医院病理科,陕西西安710038

出　　处：《现代肿瘤医学》2017年第1期17-20,共4页Journal of Modern Oncology

基　　金：国家重大基础研究计划(973计划)(编号:2015CB553703);卫生部医药卫生科技发展研究中心课题(编号:W2013FZ13);陕西省社会发展攻关计划项目(编号:2014K11-01-01-25)

摘　　要：目的:探讨DUSP10在浆液性卵巢癌组织中的表达情况及其与卵巢癌细胞迁移及侵袭能力的关系。方法:实时荧光定量PCR方法检测浆液性卵巢腺癌和正常输卵管伞端组织中DUSP10的表达情况;转染DUSP10过表达和干扰质粒至人卵巢癌A2780细胞,划痕实验和Transwell实验观察癌细胞迁移及侵袭能力的变化。结果:DUSP10在浆液性卵巢癌组织的表达低于正常输卵管伞端组织(P<0.05)。与对照组相比,转染DUSP10过表达质粒后A2780细胞迁移及侵袭能力受到抑制,转染DUSP10干扰质粒后A2780细胞迁移及侵袭能力增强。结论:DUSP10在浆液性卵巢癌组织中低表达;DUSP10可抑制人卵巢癌A2780细胞的迁移及侵袭能力。Objective： To investigate the expression of DUSP10 in serous ovarian cancer tissues and its relationship with ovarian cancer cell migration and invasion ability. Methods： Real- time quantitative PCR was performed to detect the expression of DUSP10 in serous ovarian cancer and normal fimbria of fallopian tubes. Wound healing assay and Transwell assay were used to observe the migration and invasion ability of A2780 cells by transfected DUSP10 overexpression plasmid and interference plasmid. Results： The mRNA expression of DUSP10 was significantly down-regulated in serous ovarian cancers than normal fimbria of fallopian tubes（ P〈0. 05）. The migration and invasion ability of A2780 cells were significantly inhibited after overexpression of DUSP1 0,however,silencing DUSP10 can promote cell migration and invasion. Conclusion： The expression of DUSP10 was significantly down- regulated in serous ovarian cancer,DUSP10 can inhibit the migration and invasion ability of ovarian cancer A2780 cells.

关 键 词：浆液性卵巢癌 DUSP10基因 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]