不同NaCl含量、培养基和微量元素对分枝杆菌SP-3降解菲的影响研究  

Impact of different salinity,culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3

作　　者：黄玉杰[1] 张闻[1] 傅晓文[1] 高永超[1] 孔学[1] 郭书海[1,2] 王加宁[1] HUANG Yu- jie ZHANG Wen FU Xiao-wen GAO Yong- chao KONG Xue GUO Shu-hai WANG Jia-ning(Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences,Jinan 250014, China Applied Ecology Institute, Chinese Academy of Sciences, Shenyang 110016, China)

机构地区：[1]山东省科学院生态研究所,山东省应用微生物重点实验室,山东济南250014 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《山东科学》2016年第6期98-103,共6页Shandong Science

基　　金：山东省科学院先导科技专项(2013);国家高技术研究发展计划(863计划)(2013AA06A210);泰山学者工程专项经费资助;山东省自然科学基金(BS2015HZ011)

摘　　要：研究了分枝杆菌SP-3在不同Na Cl含量、培养基和微量元素中降解菲的效果影响。结果表明,分枝杆菌SP-3在Na Cl质量分数为1%时对菲的降解率达到最大,为87.41%;菌株在LB液体培养基、改良LB液体培养基和改良的无机盐液体培养基中对菲的降解率都达到了80%以上,三者之间无显著性差异;Fe2+、Zn2+浓度分别在100μmol/L和5μmol/L,Mg2+在100μmol/L时,可以提高菌株对菲的降解能力,而Cu2+随着浓度的增加,能够抑制菌株对菲的降解。研究结果有助于该菌应用于多环芳烃降解的生物技术中,以提高菌株对菲等多环芳烃的降解能力。We addressed degradation effect of phenanthrene by Mycobacterium sp. SP-3 for different salinity, culture media and trace elements. Results show that degradation rate of phenanthrene is maximum, 87. 41%, when salt concentration is 1%. Degradation rate of phenanthrene is more than 80% in LB liquid media, modified LB and modified inorganic salt liquid media, no significant difference among them. Fe^2＋ concentration of 100 μmol/L, Zn^2＋ concentration of 5 μmol/L and Mg^2＋ concentration of 100 μmol/L can increase degradation ability of Mycobacterium sp. SP-3 to phenanthrene. However, Cu^2＋ can inhibit degradation ability of the strain to phenanthrene with the increase of its concentration. The results will be benefit for the application of the strain to biodegradation of polycyclic aromatic hydrocarbons and the increase of its biodegradation capability to polycyclic aromatic hydrocarbons such as phenanthrene.

关 键 词：菲 微量元素 培养基 降解率 分枝杆菌 

分 类 号：X172[环境科学与工程—环境科学]