雷公藤组织培养中嫩叶外植体褐化的控制  

Control of browning in the process of callus induction with Tripterygium wilfordii's new leaves

作　　者：陈凌艳[1] 陈拓[1] 吴玉香[1] 沈少炎 荣俊冬[2] 何天友[1] 郑郁善[2] 

机构地区：[1]福建农林大学园林学院,福建福州350002 [2]福建农林大学工业原料林研究所,福建福州350002

出　　处：《江苏林业科技》2016年第6期20-23,40,共5页Journal of Jiangsu Forestry Science & Technology

基　　金：国家科技支撑计划项目(2009BAI73B00);福建省中药材GAP工程技术研究中心项目(2008Y2001);福建省科技重大专项基金项目(2004YZ02-05)

摘　　要：以雷公藤当年生嫩叶为材料进行离体愈伤组织诱导培养,研究了不同处理对减轻外植体褐化率的影响。结果表明:初代愈伤组织诱导时选用液体培养基,能有效降低褐化率并延迟褐化发生时间;接种前后的特殊处理,如接种前在1.0 g/L PVP溶液中切割外植体,接种后在暗培养和冷藏条件下预处理,均能有效延迟褐化发生时间;转接时间缩短到3 d,能很好地保证愈伤组织的诱导效果,又在一定程度上降低褐化率;在培养基中加入低质量浓度(0.02 g/L)的硝酸银,能在保证有愈伤组织产出的前提下,降低雷公藤外植体的褐化。The effects of different treatments on the browning alleviation in the callus induction with Tripterygium wilfordiis new leaves were compared in this paper. The results showed as follows. Liquid medium at initial stage could delay the brow-ning happening and reduce the browning rate. Such special treatments as cutting the explants in 1. 0 g/L PVP before inoculation,treating the explants in darkness and low temperature,could delay the browning occurrence. And the browning of explants could be also decreased effectively by transferring into another medium every 3 days. Furthermore,the good anti- brow-ning effect could be got when adding 0. 02 g/L AgNO3 into medium as the healthy callus was also got as well.

关 键 词：雷公藤 组织培养 愈伤组织 诱导 褐化 总酚 

分 类 号：R282.2[医药卫生—中药学]