比沙可啶治疗慢传输型便秘大鼠的实验研究  

Experimental study of Bisacodyl in the treatment of slow transit constipation model rats

作　　者：凌杰[1] 张文忠[1] 徐斌[1] 邱伟[1] 王永兵[1] 

机构地区：[1]上海市浦东新区人民医院普通外科,201200

出　　处：《中华胃肠外科杂志》2016年第12期1365-1369,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：上海市浦东新区卫生计生委科技项目（PW2013B-6,PW2013B-7）

摘　　要：目的 探讨比沙可啶对慢传输型便秘（STC）大鼠的治疗效果及作用机制。方法 选取30只健康雌性Wister大鼠，其中20只采用复方苯乙哌啶灌胃法建立STC大鼠模型，设立STC比沙可啶组（喂饲比沙可啶）和STC对照组（普通喂饲），每组10只；剩余的10只大鼠作为空白对照组。对比空白对照组、STC对照组和STC比沙可啶组大鼠的体质量、排便粒数、粪便干重以及肠运输时间的变化，并用免疫组织化学法检测结肠Cajal间质细胞（ICC）及其c-Kit蛋白的表达。结果 喂饲复方苯乙哌啶混悬液第100天时，STC模型大鼠组较空白对照大鼠的体质量下降，粪便粒数减少，粪便干重增加，肠道传输时间延长（均P 〈 0.05），成功建立STC大鼠模型。比沙可啶治疗1个月后，空白对照组、STC对照组和STC比沙可啶组大鼠粪便粒数、粪便干重、肠道传输时间的差异有统计学意义（均P = 0.000）。其中STC比沙可啶组大鼠粪便粒数较STC对照组增多[（36.6 ± 6.8）粒/d比（26.8 ± 6.0）粒/d]，而粪便干重减少[（150.6 ± 10.5）mg/粒比（171.6 ± 16.3）mg/粒]，肠道传输时间缩短[（416.9 ± 50.6）min比（495.3 ± 66.8）min]，差异均有统计学意义（均P 〈 0.05）。STC对照组大鼠结肠壁ICC出现基膜溶解，与周围细胞间的紧密连接也有破坏，细胞核有不同程度的萎缩，单视野中数量为（8.20 ± 1.92），结肠c-Kit表达量为（12.68 ± 2.59）%，明显少于空白对照组[分别为（36.00 ± 6.25）和（71.50 ± 8.27）%]，差异均有统计学意义（均P = 0.000）。与STC对照组相比，STC比沙可啶组大鼠结肠壁ICC与周围细胞的紧密连接明显增加，ICC数量增多（18.80 ± 3.70），结肠c-Kit蛋白表达亦增加（45.91± 6.80）%，差异均有统计学意义（均P = 0.000）。结论 比沙可啶治疗可改善大鼠STC状况，其作用机制可能与增加结肠ICC数量并提高ICC内c-Kit蛋白表达有�Objective To explore the efficacy and the mechanism of Bisacodyl in treating slow transit constipation model rats.Methods A total of 30 healthy rats were enrolled. Twenty rats received intragastric diphenoxylate to develop slow transit constipation （STC） model, and 10 untreated rats were set as blank control. STC rats were subdivided into two groups： STC Bisacodyl group （fed with Bisacodyl） and STC control group （common feed） . Body weight, number and dry weight of faeces, and intestinal transit time were compared among 3 groups. Interstitial cells of Cajal （ICC） and c-Kit protein expression were measured by immunohistochemical staining.Restults Compared to blank control rats, at 100-day of receiving intragastric diphenoxylate, above 20 rats presented the decrease of body weight and feces number, the increase of dry weight of faeces, and the delay of intestinal transit time, indicating the successful establishment of STC rat model. One month after feeding, compared to STC control group, STC Bisacodyl grap had an increased feces number[ （36.6 ± 6.8） pill/day vs. （26.8 ± 6.0） pill/day], decreased dry weight of feces[ （150.6 ± 10.5） mg/pill vs. （171.6 ± 16.3） mg/pill] and shortened intestinal transit time [ （416.9 ± 50.6） minutes vs. （495.3 ± 66.8） minutes], and the differences were statistically significant （all P〈0.05） . Dissolution of ICC basement membrane, damage of connection between ICC and surrounding cells, and atrophy of ICC nucleus structure were found in STC control rats. ICC （8.20 ± 1.92 per field] and c-Kit expression （12.68 %± 2.59%） in STC control rats were significantly lower than those in blank control rats （36.00 ± 6.25 per field and 71.50 % ± 8.27%） （P = 0.000） . Compared to STC control group, the connection between ICC and surrounding cells enhanced obviously, ICC （18.80 ± 3.70 per field） and c-Kit expression （45.91% ± 6.80%） were significantly higher in STC Bisacodyl group （all P= 0.000） .Conclusion Bisacodyl t

关 键 词：慢传输型便秘 比沙可啶 CAJAL间质细胞 C-KIT蛋白 大鼠 

分 类 号：R574.62[医药卫生—消化系统]