结直肠癌患者赖氨酰氧化酶检测的临床意义  

Clinical significance of determination of the serum levels and the expression of LOX in patients with colorectal cancer

作　　者：杨永宾[1] 杨洁[2] 张红鹏 白海岭 石晓明[1] 

机构地区：[1]河北省人民医院普外二科,石家庄050051 [2]河北省人民医院血液科,石家庄050051 [3]邯郸县人民医院外科,河北邯郸056005

出　　处：《重庆医学》2016年第35期4971-4973,共3页Chongqing medicine

摘　　要：目的 探讨赖氨酰氧化酶（LOX）在结直肠癌（CRC）患者组织及外周血中的表达情况,并分析其关系和临床意义。方法 选取2014年1~12月于河北省人民医院行CRC切除术的96例患者作为CRC组,其中行根治性手术者74例（根治组）,非根治性手术者22例（非根治组）;同时选取体检健康者60例作为对照组。取CRC患者肿瘤及癌旁正常黏膜组织,采用免疫组织化学染色检测LOX表达;采用酶联免疫吸附试验（ELISA）检测各组外周血LOX、癌胚抗原（CEA）、糖链抗原19-9（CA19-9）表达水平。结果 CRC组织中LOX阳性率（75.00%）高于癌旁正常黏膜（26.04%）,且非根治组肿瘤组织LOX阳性率（95.45%）高于根治组（68.92%）,差异均有统计学意义（P〈0.05）。CRC患者外周血LOX、CEA及CA19-9水平均高于对照组,非根治组患者外周血LOX水平高于根治组,差异均有统计学意义（P〈0.05）。CRC患者肿瘤组织LOX表达水平与外周血LOX水平呈正相关（r=0.75,P〈0.05）。CRC患者外周血LOX与CEA水平呈正相关（r=0.42,P〈0.05）;CRC患者外周血LOX与CA19-9水平无明显相关性（r=0.12,P〉0.05）。结论 CRC患者外周血及肿瘤组织中LOX表达水平增高,且与肿瘤转移有关,检测外周血LOX水平对预测CRC转移有意义。Objective To explore the expression status of lysyl oxidase（LOX） in tumor tissues and peripheral serum of patients with colorectal cancer（CRC） ,and to analyse their relationships and clinical significance. Methods A total of 96 cases of patients with CRC who underwent surgical resection of CRC in Hebei General Hospital were enrolled into the CRC group, among them,there was 74 cases of patients underwent radical surgery（radical surgery group）,while the other 22 cases of patients underwent palliative surgery（palliative surgery group）. Simultaneously, 60 cases of healthy individuals were enrolled into the control group. The colorectal cancer tissues and the adjacent normal tissue were collected from patients with CRC,and the immunohistochemical technique was performed to detect the expression of LOX. The peripheral serum levels of LOX, CEA and CA19-9 were detected by using ELISA in 96 cases of patients with CRC and 60 healthy volunteers. Results The positive rate of LOX expression in eolorectal cancer tissues（75.00 %） was significantly higher than that in adjacent normal tissues（26.04%）, and the positive rate of LOX expression in the palliative surgery group（95.45 %） was significantly higher than that in the radical surgery group（68.92 %）, there were statistically significant differences（P〈0.05）. The peripheral serum levels of LOX, CEA and CA19-9 in patients with CRC were significantly higher than those in the Control group,and the peripheral serum levels of LOX in he radical surgery group was significantly higher than that in the palliative surgery group, there were statistically significant differences（P〈0.05）. In patients with CRC, the expression level of LOX in CRC tissue was positively correlated with that in peripheral serum （r= 0.75, P〈 0.05）. There was positive correlation between peripheral serum LOX and CEA in patients with CRC（r= 0.42, P〈0.05）, while no significant correlation was found between peripheral serum LOX and CA19-9（r=0. 12, P〈0. 05）.

关 键 词：结直肠癌 赖氨酰氧化酶 癌胚抗原 转移 

分 类 号：R735.34[医药卫生—肿瘤]