高迁移率族蛋白-1及Hepsin在宫颈癌组织中的表达与临床意义  

Expression and clinical significance of high mobility group box-1 and Hepsin in cervical carcinoma tissues

作　　者：程慧[1] 张蓓[1] 成杰[1] 李妍雨 滕鹏[1] 

机构地区：[1]徐州市中心医院妇产科,江苏221009

出　　处：《中华妇幼临床医学杂志（电子版）》2016年第6期660-666,共7页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：江苏省医学重点人才基金项目(RC2011047)~~

摘　　要：目的 探讨高迁移率族蛋白（HMGB）-1和Hepsin在宫颈癌中的表达与临床意义。方法 选择2008年5月至2010年6月,在徐州市中心医院住院治疗的70例宫颈癌患者为研究对象。将所有患者手术切除的宫颈癌组织及癌旁2cm处组织,分别纳入宫颈癌组及癌旁组,均为70例;并选取同期因宫颈良性病变于本院手术获取的20例良性病变宫颈组织,纳入对照组。采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶链结（SP）法检测上述3组组织中HMGB-1及Hepsin蛋白表达情况,并统计学分析其表达差异,不同临床因素下HMGB-1及Hepsin蛋白在宫颈癌组织中阳性表达率差异及此2种蛋白在宫颈癌组织中表达的相关性;并对2种蛋白不同阳性表达强度宫颈癌患者进行生存分析。本研究所有研究对象均签署知情同意书,并取得本院伦理道德委员会批准。结果 ①宫颈癌组HMGB-1及Hepsin蛋白阳性表达率,均分别较癌旁组或对照组高,而癌旁组此2种蛋白阳性表达率,均较对照组高,且差异均有统计学意义（P〈0.05）。②免疫组织化学SP染色结果显示,良性病变宫颈组织中HMGB-1及Hepsin蛋白表达呈阴性,而宫颈癌癌旁组织中此2种蛋白呈淡黄色或黄色阳性（＋＋）表达,宫颈癌组织中,则此2种蛋白呈棕褐色强阳性表达（＋＋＋）,并且主要位于细胞质。③宫颈癌组的70例宫颈癌组织中,肿瘤浸润深度为T3＋T4者及有淋巴结转移者的HMGB-1蛋白阳性表达率,较T1＋T2者及无淋巴结转移者高,且差异均有统计学意义（P〈0.05）;而肿瘤分化程度为低分化者、肿瘤浸润深度为T3＋T4者、有淋巴结转移者,以及肿瘤、淋巴结转移、远隔器官转移（TNM）分期为Ⅲ＋Ⅳ期者的Hepsin蛋白阳性表达率,均较肿瘤分化程度为高/中分化者、肿瘤浸润深度为T1＋T2者、无淋巴结转移者及肿瘤TNM分期为Ⅰ＋Ⅱ期者高,且差异均有统计学意义（P〈0.05）。④HMGObjective To explore expression and clinical significance of high mobility group box （HMGB）-1 and Hepsin in cervical carcinoma. Methods A total of 70 cases of cervical carcinoma patients from May 2008 to June 2010 in Xuzhou Central Hospital were selected as study subjects. Seventy cases of surgical ablated cervical carcinoma tissues and 70 cases of para-cancer tissues of 2 cm near to cancer, were included in cervical carcinoma group and para-cancer group; and 20 cases of benign lesion cervical tissues which were obtained from cervical tumor enucleation or adnexectomy because of benign cervical lesions in the same period in same hospital were selected as the control groups. The expression of HMGB-1 and Hepsin proteins in these 3 groups were detected by immunohistochemistry streptavidin-perosidase （SP） method. The differences of expression levels of HMGB-1 protein and Hepsin protein in 3 groups and the differences of positive expression rates of HMGB-1 and Hepsin proteins in cervical carcinoma tissue in different clinical factors were compared statistically. Analyzed the correlation between HMGB-1 protein and Hepsin protein expression level in cervical carcinoma tissue. And the survival analysis of cervical carcinoma patients with different expression intensities of 2 kinds of proteins were conducted. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Xuzhou Central Hospital. Informed consent was obtained from each participant. Results （1）The positive expression rates of HMGB-1 and Hepsin proteins in cervical carcinoma group were higher than those of para-cancer group and control group, respectively,also the positive expression rates of HMGB-1 and Hepsin proteins in para-cancer group were higher than those of control group, and the differences were statistically significant （P〈0.05）. （2）Imrnunohistochemical SP staining results showed that the HMGB-1 and Hepsin proteins were negative expression in benign lesion cervical tissues, pale yellow or yellow

关 键 词：宫颈肿瘤 HMGB1蛋白质 HEPSIN 肿瘤侵润 女性 

分 类 号：R737.33[医药卫生—肿瘤]