用复合诱导型骨修复材料修复兔桡骨缺损的实验研究  

An Inducible Bone Repair Composite Material PHBV/CS:The Renovation of Rabbit Radius

作　　者：李根[1] 甄平[1] 成满平[1] 赵红斌[1] 

机构地区：[1]兰州军区兰州总医院,兰州730050

出　　处：《中国生物医学工程学报》2016年第6期719-728,共10页Chinese Journal of Biomedical Engineering

基　　金：甘肃省科技重大专项(1203FKDA036)

摘　　要：以不同的载药方式构建4种壳聚糖/聚羟基丁酸酯-羟基戊酸酯（PHBV）复合诱导型骨修复材料,检测并比较4种支架材料对兔桡骨缺损的修复效果,筛选出最佳骨修复材料并确定最佳药物控释方式。以淫羊藿苷为诱导因子,采用两相混合冷冻干燥技术以微球载药、改性药物微球（W/O法制得并表征）、改性药物与材料共价结合等药物添加方式及不加药制得4种支架材料,并对其进行显微结构以及载药支架药物缓释表征,后将4种材料分别植入兔桡骨缺损处,于1、3、6个月进行X射线及三维CT观察支架材料对兔桡骨缺损的修复情况,HE,Masson染色观察其诱导成骨效果。结果表明,支架材料呈网络状串珠状的显微结构,载药微球粒径分布在3-11μm,载药支架材料有着良好的药物缓释,其中共价结合组药物释放峰值时间较其他组推迟,为72 h,且峰值后药物缓释量迅速平稳为75μg左右。X射线及三维CT观察显示,最终共价结合组支架材料骨缺损处连通,且骨密度高于其他3组。HE、Masson染色结果显示,共价结合组成骨效果优于其他组。共价结合的药物添加方式能使支架具有良好的药物缓释效果,进而对兔桡骨缺损表现出良好的修复效果。In this work, four kinds of chitosan/poly hydroxybutyrate-co-hydroxyvalerate （PHBV）composite scaffold were fabricated by the method of two phase mixture freeze drying. The repair effects of the scaffoldswere investigated and compared to determine the optimal formula for guiding bone regeneration as well as drug releasing. The icariin （Ica） was used in this study as an osteoinductive factor, and Ica microspheres were fabricated by W/O method and covalent binding. Microstructures of the scaffolds and drug releasing behaviors were investigated. After that,the scaffolds were implanted in the radius defects of rabbits;X-ray and 3D CT were applied to observe the repair effect at 1, 3, and 6 months post surgery. The osteogenesis inducible effects were evaluated using HE and Masson staining. The scaffolds showed network-like microstructures with particles inside, the drug loaded particles was 3 - 11 μm in diameter. The scaffolds released Ica well, though the peak of drug release curve for the covalent binding group was delayed than the others. The peak appeared at 72 h after immersion and quickly reached a stabled level of 75 μg. The images of X-ray and 3 - D CT showed the defect of the rabbit radius had connected and the bone mineral density was higher than the other three groups. HE and Masson stained sections of the radius segmental defect of rabbits implanted for 1, 3, and 6 months showed that the scaffold with covalently binding Ica had better repair effect than the others.

关 键 词：聚羟基丁酸酯-羟基戊酸酯 诱导 共价结合 骨修复 

分 类 号：R318.08[医药卫生—生物医学工程]