STAT3/iNOS信号对喉癌Hep-2细胞的增殖作用  

The proliferation effect of STAT3/iNOS signaling pathway in laryngeal cancer Hep-2 cell line

作　　者：王莹[1] 李文媛[1] 赵晨辉 金在顺[1] 刘艳翠[1] 张洋[1] 张晶[1] 

机构地区：[1]牡丹江医学院解剖教研室 [2]牡丹江市第二人民医院五官科,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2016年第6期11-13,10,共4页Journal of Mudanjiang Medical University

基　　金：牡丹江市科学技术计划项目(Z2014s035);牡丹江医学院科学技术研究项目(2s201331)

摘　　要：目的观察信号转导及转录激活蛋白3(STAT3)/诱导型一氧化氮合酶(iNOS)信号通路对喉癌Hep-2细胞(Hep-2)的增殖作用,并探讨其作用机制。方法体外培养Hep-2细胞,STAT3慢病毒(2μL,1×1010病毒颗粒)和同剂量siRNA STAT3转染Hep-2细胞6 d后,细胞分为对照组、siRNA STAT3组和STAT3组。MTT法检测各组Hep-2细胞增殖情况。实时荧光定量PCR检测检测各组细胞STAT3、iNOS及血管内皮因子C(VEGF-C)mRNA相对表达。结果镜下观察STAT3组Hep-2细胞数量显著高于对照组和siRNA STAT3组,MTT法显示STAT3组Hep-2细胞在6 d增殖显著高于对照组和siRNA STAT3组(P<0.05),实时荧光定量PCR结果表明STAT3转染组STAT3、iNOS和VEGF-C mRNA表达显著高于对照组和siRNA STAT3组(P<0.05),而对照组和siRNA STAT3组STAT3、iNOS和VEGF-C蛋白表达未见显著差异(P﹥0.05)。STAT3与iNOS及VEGF-C mRNA表达显著正相关(P<0.05)。结论 STAT3/iNOS信号通路能够通过上调VEGF-C表达促进喉癌Hep-2细胞增殖,STAT3/iNOS信号通路可成为治疗喉癌新的靶点。Objective To observe the proliferation effect of STAT3/iNOS signal in Hep - 2 cell line, and to investigate the mechanism. Methods Hep- 2 cell were cultured in vitro, STAT3 lentivirus （2 μl,1 × 10^10 viral particles） and the same dose of siR- NA STAT3 transfection Hep - 2 for 6 d, cells were randomly divided into control group, siRNA STAT3 group and STAT3 group. The proliferation of Hep -：2 call was detected by MTT method, STAT3, iNOS and VEGF - C mRNA expression in Hep -2 cell were detected with by PCR, Results Hep - 2 cell density in STAT3 group was significantly higher than that of control group and STAT3 siRNA group under microscope. MTT assay showed Hep - 2 cell proliferation in STAT3 group was significantly higher than that of control group and STAT3 siRNA group on fi days （ P 〈 0.05 ）, PCR results show that STAT3, iNOS and VEGF - C mRNA expression in STAT3 group was significantly higher than that of the control group and STAT3 siRNA group （ P 〈 0.05 ）, while the STAT3, iNOS and VEGF - C protein expression in control group and siRNA STAT3 had no significant difference （ P 〉0. 05 ）. Findings demonstrated a significantly positive correlation between STAT3 mRNA, iNOS mRNA and VEGF - C mRNA （P 〈 0.05）. Conclusion STAT3/ iNOS signaling pathway can promote the proliferation of Hep - 2 cells by upregulating the expression of VEGF - C, STAT3/iNOS signa- ling pathway may become the new targets of tumor lymphangiogenesis.

关 键 词：喉癌HEP-2细胞 转录活化因子3 诱导型一氧化氮合酶 增殖 

分 类 号：R73[医药卫生—肿瘤]